Instituto Politécnico de Viseu

Scientific Repository

Welcome to the Polytechnic Institute of Viseu Institutional Repository

The aim of this Repository is to give greater visibility to the scientific production of the academic community of the Polytechnic Institute of Viseu, by increasing the impact and use through Open Access, ensuring the storage and preservation of all research produced in its organic units and research centres.

Tel +351 232 419 035

Email repositorio@sc.ipv.pt

Recent Submissions

CIRCULATING BETA-TRACE PROTEIN VERSUS CREATININE AS MARKERS OF EARLY KIDNEY (DYS)FUNCTION IN THE NX-INDUCED RAT MODEL OF CHRONIC KIDNEY DISEASE

Publication . Lousa, Irina; Belo, Luis; Viana, Sofia D.; Vieira, Pedro; Vala Correia, Helena Maria; Santos-Silva, Alice; Reis, Flavio

2023-07-18Conference paper

Open access

CHARACTERIZATION OF RENAL (DYS)FUNCTION IN RAT MODELS OF EARLY AND ADVANCED CHRONIC KIDNEY DISEASE

Publication . Lousa, Irina; Reis, Flávio; Vieira, Pedro; Vala Correia, Helena Maria; Belo, Luís Filipe Barbosa Amado; Santos-Silva, Alice

2023-08Conference poster not in proceedings

Restricted access

Estrongilose pulmonar em pequenos ruminantes: uma doença subestimada?

Publication . Coelho, Catarina; Teresa Letra Mateus; Vila-Viçosa, Maria João; Arede dos Santos, Carla Sofia; Esteves, Fernando; Esteves, Fernando; Cruz, Rita; Gomes, Liliana; Henriques, Diogo; Vala Correia, Helena Maria; et.al

2023Conference object

Restricted access

Hepatocellular Early Apoptosis Associated with HES 130/0.4 Administration for Volume Replacement in Pigs After Severe Bleeding

Publication . Vala, Helena; Faustino-Rocha, Ana I.; Cruz, Rita; Venâncio, Carlos; Silva, Aura; Mesquita, João R.; Ortiz, Ana Liza; Ferreira, David A.

Hydroxyethyl starch (HES) 130/0.4 is commonly used for volume replacement, yet its hepatic effects in the context of acute haemorrhage remain unclear. This study aimed to evaluate hepatic histopathological changes related to HES 130/0.4 administration when compared to Ringer’s lactate (RL) in healthy pigs subjected to acute bleeding under general anaesthesia. Eighteen pigs were randomised into three groups: RL (n = 6), HES 130/0.4 (n = 6), and a non-bleeding control (n = 6). Liver tissue was collected postmortem and analysed using haematoxylin–eosin staining, cytochrome c immunohistochemistry, the TUNEL assay, and M30 immunofluorescence. No statistically significant differences were observed in general histopathological changes, TUNEL, or cytochrome c expression (p > 0.050). However, the pigs that received HES 130/0.4 for volume replacement showed significantly higher intensity of the liver M30 immunostaining in the Q-score (p < 0.010), H-score (p < 0.010), and c indexc index (p < 0.050) when compared to animals that received Ringer’s lactate solution or animals in the control group. These findings suggest that HES 130/0.4 induces increased early hepatocellular apoptosis when compared to RL in this model, raising concerns about its hepatic safety profile under haemorrhagic conditions.

2025-08-22Research article

Open access

Hepatocellular Early Apoptosis Associated with HES 130/0.4 Administration for Volume Replacement in Pigs After Severe Bleeding

Publication . Vala Correia, Helena Maria; Ana I. Faustino-Rocha; Rita Cruz; Carlos Venâncio; Aura Silva; João R. Mesquita; Ana Liza Ortiz; David A. Ferreira; Cruz, Rita

2025-08-22Research article

Open access