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Advisor(s)
Abstract(s)
Bovine Ocular Squamous Cell Carcinoma (OSCC) is the second most common cause of rejection due to neoplasia in slaughterhouses on S. Miguel Island, Azores and accounts for significant economic losses. In order to obtain better insight into the genesis and neoplastic transformation process of bovine OSCC, abnormal protein expression and proliferation index were assessed by the immunohistochemical evaluation of p53 and Ki67, respectively. OSCC samples were collected from fifteen bovine, and were classified histologically according to the degree of differentiation in three categories: poorly, moderately and well differentiated. Immunohistochemistry using polyclonal anti-human p53 antibody and polyclonal anti-human Ki67 antibody was performed. Ten out of fifteen tumours tested were immunoreactive for p53. Twelve tumours demonstrated Ki67 expression. As in human squamous cell carcinoma, p53 overexpression is frequent in bovine OSCC, providing support for a possible role of the protein in the pathogenesis of this neoplasia. No correlation between the percentage of p53 stained nuclei and the degree of differentiation was observed, although different patterns of staining were seen according to the degree of keratinization of the tumour cells. With the exception of moderately differentiated OSCC group, Ki67 index showed significant correlation with the histological pattern, increased proliferation being found in poorly differentiated OSCC (P=0.013).
Description
Keywords
Bovine Cattle Immnunohistochemistry Ocular Squamous Cell Carcinoma Ki67 p53
Citation
Carvalho T, Vala H, Pinto C, Pinho M, Peleteiro C (2005). Immunohistochemical studies of epidermal cell proliferation and p53 mutation in Bovine Ocular Squamous Cell Carcinoma. Veterinary Pathology. 42: 66-73