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Advisor(s)
Abstract(s)
Introduction: Type 2 diabetes mellitus 2 (T2DM) results, amongst others, from insulin resistance and reduction of ß-cell secretion, produced by functional changes and cellular mass reduction.
Aim: Chronological morphologic characterization of the endocrine pancreas in an animal model of T2DM, the Zucker Diabetic Fatty (ZDF) rats to elucidate the underlying mechanisms of disease evolution.
Material and methods: The pancreas of diabetic, obese rats (ZDF Gmi fa/fa) and their littermates (ZDF Gmi +/+) were compared at 8, 20, 26 weeks of age. After routine stain haematoxylin-eosin. Quantitative and semi-quantitative parameters were applied for evaluation of morphological changes in the islets of Langerhans. Quantitative parameters included counting islet numbers and islet size measurement. Semi-quantitive parameters consisted in the evaluation of islet conformation and perimeter regularity. The inflammatory lesions, vascular congestion and β-cell vacuolization were also classified in a scale from 0 to III.
Results: Obese, diabetic ZDF (fa/fa) rats presented, with increasing age, a reduction in islet numbers, progressive deformation of insular architecture, fibrosis and vascular congestion, connected with inflammatory changes and joined by a decrease in insular cellular mass.
Conclusion: The coincidence of the progressive histopathologic changes with the metabolic profile obtained in our previous studies shows a decrease in the capacity of the endocrine pancreas to compensate insulin resistance and ß-cell dysfunction. These two conjunct facts make the ZDF fa/fa, rat an appropriate model for a variety of T2DM studies.
Description
Keywords
Type 2 diabetes mellitus 2 (T2DM) morphologic characterization of the endocrine pancreas Zucker Diabetic Fatty (ZDF) rats animal model of T2DM
Citation
Mega C, Vala H, Teixeira de Lemos E, Fernandes R, Garrido P, Sereno J, Parada B, Melo F, Teixeira F, Reis F (2010). Utilização de parâmetros quantitativos e semi-quantitativos para avaliação dos Ilhéus de Langerhans num modelo animal de Diabetes Mellitus tipo 2. Revista Portuguesa de Ciências Veterinárias. Supl. 109(573-576): 82.