Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.19/1498
Título: Immunohistochemical studies of epidermal differentiation, cell proliferation and p53 mutation in bovine ocular squamous cell carcinoma
Autor: Vala, Helena
Afonso, A. F.
Esteves, F.
Santos, C.
Seixas, C.
Amaral, M.
Albuquerque, T.
Botelho, A.
Amado, A.
Palavras-chave: Bovine Ocular Squamous Cell Carcinoma
BOSCC
immunohistochemistry
histopathological diagnosis
Ki67
p53
Data: 2002
Citação: Vala H, Fondevila D, Carvalho T, Pinto C, Peleteiro C, Pinho M, Ferrer L. 2002. Immunohistochemical studies of epidermal differentiation, cell proliferation and p53 mutation in bovine ocular squamous cell carcinoma. Proceedings of 20th Meeting ESVP: 4
Resumo: The purpose of the present study was to immunocharacterize Bovine Ocular Squamous Cell Carcinoma neoplastic cells, in terms of proliferation index and DNA damage, using two human primary antibodies anti- Ki67 antigen of the cell cycle and anti- p53. Ki67 is a nuclear antigen expressed in dividing cells during late G1, G2, S-phase and M-phase that disapears rapidly after mitosis. p53 is a tumor supressor protein that plays a key role in the control of the DNA damage acting as a supressor of tumour cell proliferation, enhancing genetic stability. Mutation of the gene that codes for p53 provides an extended intracellular half-life of the protein and greater stability, therefore increasing its expression. Tumours for this study were obtained at the slautherhouse, in Ponta Delgada, Azores. A total of thirty samples were colected from eighteen bovine. Immunohistochemical analysis was performed in parafin embeded sections of formalin fixed material, using the Avidin-Biotin-Peroxidase method (ABC). Primary antibodies used were anti-human p53 (NCL – p53 CM-1, NOVOCASTRA, UK) and anti-human Ki67 (NCL – Ki67p, NOVOCASTRA, UK), both policlonal. Positivity for both Ki67 and p53 was evaluated quantitatively and semi-quantitatively, respectively.
Peer review: yes
URI: http://hdl.handle.net/10400.19/1498
Aparece nas colecções:ESAV - DZERV - Documentos de congressos (comunicações, posters, actas)

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
9 Vala et al Ep Diff p53 2002.pdf1,39 MBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.