Browsing by Author "Faustino-Rocha, AI"
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- Effects of intense pulsed light on skin carcinogenesisPublication . Faustino-Rocha, AI; Gama, A.; Rodrigues, D.; Colaço, B.; Vala, Helena; Pires, MJ; Oliveira, PAObjective: Intense pulsed light (IPL) is a non-laser high intensity light that has been used to the treatment of several skin conditions. This work intended to evaluate the effects of IPL on a mouse model of two-stage skin carcinogenesis. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Sixteen DBA/2JRccHsd mice were divided into two groups: IPL-exposed and non-exposed. The carcinogen agent DMBA was topically applied once and TPA was applied twice a week during 22 weeks. Animals IPL-exposed received IPL applications twice a week, at an intensity of 2J/cm2 during 22 weeks. Animals were sacrificed and skin samples were histologically evaluated. Results: Animals IPL-exposed developed 44 skin lesions (5.5 lesions per animal) while non-exposed animals developed 63 lesions (7.9 lesions per animal) (p=0.066). The animals IPL-exposed developed 20 skin neoplastic lesions while non-exposed animals developed 38 lesions (p=0.018), however the number of malignant lesions was higher in IPL-exposed animals (3 lesions) than in non-exposed animals (1 lesion) (p>0.05). Conclusion: The animals IPL-exposed developed lower number of skin lesions and neoplastic lesions, but the number of malignant lesions was higher. IPL inhibits the development of skin lesions but may have a promoting effect on the malignant conversion.
- Implementation of humane endpoints on bladder cancer researchPublication . Oliveira, MC; Faustino-Rocha, AI; Vala, Helena; Oliveira, PAObjective: Pain, distress, or discomfort should be minimized during experimental procedures using animals. The goal of this work was to establish humane endpoints for a model of bladder cancer chemically-induced by N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN). Methods: Procedures were made in accordance with the European Directive 2010/63/EU. Twelve Wistar rats were used. The carcinogen agent BBN was administered in drinking water during 20 weeks. After this, they consumed only water until 35 weeks of the experimental protocol. During the protocol was used a scoring sheet where biological variables were registered daily to monitor animal welfare. Results: No animals died during the experimental protocol. Four animals showed signs of haematuria since the fifteenth week after the beginning of the experiment, these animals also exhibited moderate anemia in the last week of the protocol that was evident through the mucosal observations. All animals exhibited a mental status, eyes, ears and whiskers position, response to handling, breathing and hydration within the parameters considered normal for the specie. Conclusion: The animals did not show clinical signs compatible to suffering that justified their sacrifice before the end of the study. The endpoints established for this model are adequate to bladder cancer studies once no endpoints were reached.