Publication
Optimized Production of Fungal Polygalacturonase Using Cupuaçu (Theobroma grandiflorum) Peel as Substrate and Its Effect on Clarification of Cupuaçu Juice
| datacite.subject.fos | Ciências Agrárias | |
| datacite.subject.sdg | 12:Produção e Consumo Sustentáveis | |
| dc.contributor.author | Falcão, Lucas | |
| dc.contributor.author | Monteiro, Trisha | |
| dc.contributor.author | Azevedo, Sthéfanny | |
| dc.contributor.author | Batista, Bárbara | |
| dc.contributor.author | Jordão, António | |
| dc.contributor.author | Albuquerque, Patrícia | |
| dc.date.accessioned | 2026-01-05T09:33:12Z | |
| dc.date.available | 2026-01-05T09:33:12Z | |
| dc.date.issued | 2025-01-01 | |
| dc.description.abstract | Pectinolytic enzymes play a key role in many beverages manufacturing processes, improving their clarification and filtration steps. Fungal pectinases are considered promising green catalysts for industrial applications, and they can be produced using fruit-processing residues as substrate. In this study, we investigated the optimal conditions to produce polygalacturonase from Aspergillus brasiliensis in a solid-phase bioprocess, using cupuaçu (Theobroma grandiflorum) peel as substrate. Then, the pectinolytic extract was applied in the clarification of cupuaçu juice. A central composite design was used to determine the optimal fungal cultivation conditions. Thus, the optimal fungal cultivation (maximum production of 11.81 U/g of polygalacturonase) was obtained using cupuaçu peel with 80% moisture, at 34 ◦C, for 7 days in a medium containing 4.2% phosphorus and 2.6% nitrogen. The enzymatic extract showed greater activity at 60 ◦C and stability at a pH range between 5.0 and 7.0. The pectinolytic extract was able to clarify the cupuaçu juice, causing a 53.95% reduction in its turbidity and maintaining its antioxidant activity. Our results demonstrate that the cupuaçu peel can be used as a substrate to produce polygalacturonase, and the enzymatic extract produced can be applied in the cupuaçu juice processing, contributing to the circular economy. | eng |
| dc.identifier.citation | Falcão, L.d.S.; Monteiro, T.E.d.A.; do Amaral, T.S.; Azevedo, S.C.M.; Batista, B.N.; Jordão, A.M.; Albuquerque, P.M. Optimized Production of Fungal Polygalacturonase Using Cupuaçu (Theobroma grandiflorum) Peel as Substrate and Its Effect on Clarification of Cupuaçu Juice. Beverages 2025, 11, 6. | |
| dc.identifier.doi | 10.3390/beverages11010006 | |
| dc.identifier.uri | http://hdl.handle.net/10400.19/9604 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | fungal pectinase | |
| dc.subject | agro-industrial residues | |
| dc.subject | cupuaçu juice | |
| dc.subject | antioxidant activity | |
| dc.subject | clarification | |
| dc.title | Optimized Production of Fungal Polygalacturonase Using Cupuaçu (Theobroma grandiflorum) Peel as Substrate and Its Effect on Clarification of Cupuaçu Juice | eng |
| dc.type | dataset | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 6 | |
| oaire.citation.title | Beverages | |
| oaire.citation.volume | 11 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Jordão | |
| person.givenName | António | |
| person.identifier.ciencia-id | 1212-F02A-B14D | |
| person.identifier.orcid | 0000-0003-1129-4633 | |
| person.identifier.scopus-author-id | 34770030700 | |
| relation.isAuthorOfPublication | 32c0ef5c-f36b-4810-b994-e14eea9fa04f | |
| relation.isAuthorOfPublication.latestForDiscovery | 32c0ef5c-f36b-4810-b994-e14eea9fa04f |