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Advisor(s)
Abstract(s)
Being Paratuberculosis a chronic disease of difficult diagnosis and having suspicious of her presence in Serra da Estrela ovine’s effectives, it’s essential to prove this fact and establish a fast, efficient and viable diagnosis protocol, to be able to identified flock’s carriers of disease cases and, subsequently, being developed an eradication plane that allows her control.
Therefore, in this work were tested several diagnosis’ methods but this report reflects mostly on the histopatological diagnosis’ methods, including the general condition observation before necropsy elaboration, necropsy, histopathological exam and Ziehl-Neelsen’s stain method, as well as Immunohistochemical`s method. A analogy with serological diagnosis was made.
Of the 46 animals sent to necropsy, 20 showed positive reaction to ELISA (enzyme linked immunosorbent assay), 2 doubtful reaction and 21 were negative. The AGID was only positive in 4 animals. The symptoms were compatible in 34 animals. The macroscopic’s exam was compatible in 40 animals. The histopathology was compatible in 26 animals. The Ziehl-Neelsen’s stain of tissues revealed acid fast bacterias in 20 animals. In Immunohistochemical method 20 animals were positives.
Description
Keywords
Paratuberculosis Serra da Estrela ovine histopatological diagnosis necropsy Ziehl-Neelsen’s stain method Immunohistochemistry ELISA AGID
Citation
Vala H, Santos C, Eteves F, Albuquerque T, Afonso A, Botelho A, Seixas C, Amaral M, Amado A (2007). Paratuberculosis in Sheep from Serra da Estrela Region, Portugal. Paratuberculosis: 252-255