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Hepatic histopathological lesions in acute controled haemorrhage followed by volume replacement with a crystalloid or colloid solution

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dc.contributor.authorVala, Helena
dc.contributor.authorPina, R.
dc.contributor.authorCruz, R.
dc.contributor.authorVenâncio, C.
dc.contributor.authorEsteves, Fernando
dc.contributor.authorSilva, A.
dc.contributor.authorMesquita, João
dc.contributor.authorOrtiz, A. L.
dc.contributor.authorFerreira, D.
dc.date.accessioned2017-11-29T13:29:47Z
dc.date.available2017-11-29T13:29:47Z
dc.date.issued2017
dc.description.abstractIntroduction: severe hemorrhage remains the major cause of morbidity and mortality in trauma victims or surgical intervened animals, despite of all the advances in the therapeutic approach. The resulting injuries, or even death, are due to the deficit in intravascular volume and subsequent development of hypovolaemic fluid state, leading to poor tissue perfusion and consequent decreased oxygen delivery to the tissues, often with compromise of organ function. Objective: the aim of this work was to evaluate the effect of different intravenous solutions used for volume replacement following acute controlled haemorrhage, one crystalloid (RL) and one colloid (HES130/0.4) in the integrity of the hepatic tissue in a pig animal model. Material and methods: hepatic samples were collected from animals submitted to passive arterial blood bleeding and reperfusion with a crystalloid (RL) (G1) and with a synthetic colloid (HES 130/0.4) (G2). Samples were also collected from animals that were not subjected to acute bleeding nor volume replacement (G3, control group). All procedures were carried out under personal and project licenses approved by the Ethical Committee of the national regulatory office. Samples were collected and fixed in 10% neutral-buffered formalin, for a maximum of 24hours, embedded in paraffin wax and 3µm sections were stained for routine histopathology with haematoxylin and eosin. Results: the histopathological assessment revealed no statistically significant differences between the three groups. However, some lesions were more often expressed in some groups. More severe hepatocellular hydropic degeneration and hepatocellular steatosis was seen in G1, which is the only group in which haemorrhage was observed and within which oedema was not present. Hyperaemia was only observed in G2 and G3. Necrosis was not present in any of the groups. Conclusion: hepatic histopathological lesions following controlled bleeding and intravenous volume replacement with RL or HES130/0.4 were subtle. However, more pronounced hydropic degeneration and hepatocellular steatosis was seen in G1 (RL), which suggests that HES130/04 may be associated with better hepatic perfusion when used for intravenous volume replacement when compared to RL.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVala, H., Pina, R., Cruz, R., Venancio, C., Esteves, F., Silva, A., ... Ferreira, D. (Agosto/Setembro, 2017). Hepatic histopathological lesions in acute controled haemorrhage followed by volume replacement with a crystalloid or colloid solution (pp. 67-68). In Abstract book of the Cutting Edge Pathology, 3rd Joint European Congress of the ESVP, ESTP and ECVP, Lyon, France.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/4726
dc.language.isoengpt_PT
dc.relationThis work is financed by national funds through FCT - Fundação para a Ciência e Tecnologia, I.P., under the project UID/Multi/04016/2016. Furthermore we would like to thank the Instituto Politécnico de Viseu, CI&DETS for their support and FCT, FCOMP-01-0124-FEDER-009525; IPV, CI&DETS, FCT and QREN/FEDER (Ovislab ICT-2013-05-004-5314 ID-64757). This work is supported by European Investment Funds by FEDER/COMPETE/POCI– Operacional Competitiveness and Internacionalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013. Furthermore we would like to thank to UTAD and CITABS for their support.pt_PT
dc.subjectHepatic histopathological lesionspt_PT
dc.subjectAcute controled haemorrhagept_PT
dc.subjectColloid solutionpt_PT
dc.subjectCrystalloid solutionpt_PT
dc.subjectVolume replacementpt_PT
dc.titleHepatic histopathological lesions in acute controled haemorrhage followed by volume replacement with a crystalloid or colloid solutionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLyon, Francept_PT
oaire.citation.endPage68pt_PT
oaire.citation.startPage67pt_PT
oaire.citation.titleCutting Edge Pathologypt_PT
person.familyNameVala Correia
person.familyNameEsteves
person.familyNameMesquita
person.givenNameHelena Maria
person.givenNameFernando
person.givenNameJoão
person.identifierB-6960-2016
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.ciencia-id6E19-A3CC-897E
person.identifier.ciencia-idCB1F-AFD2-529E
person.identifier.orcid0000-0001-6829-4867
person.identifier.orcid0000-0003-0589-0746
person.identifier.orcid0000-0001-8769-8103
person.identifier.scopus-author-id35345458100
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication93d688a9-9716-444c-9d08-fdca593d630d
relation.isAuthorOfPublicationffcea03f-5943-43ae-aca6-a1b93b2b6134
relation.isAuthorOfPublication.latestForDiscovery93d688a9-9716-444c-9d08-fdca593d630d

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