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Comparative Analysis of Secondary Metabolite Production by Cophinforma mamane and Aspergillus niger Preserved for Long-Term Methods

2025-11-27Dataset Open access
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datacite.subject.fosCiências Agrárias
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorPadrón-Antonio, Yuliana
dc.contributor.authorFalcão, Lucas
dc.contributor.authorSouza, Anne
dc.contributor.authorAndrade, Cleudiane
dc.contributor.authorSilva, Paulo
dc.contributor.authorBarbosa, Larissa
dc.contributor.authorJr, Sérgio
dc.contributor.authorCorreia, Ana Cristina
dc.contributor.authorJordão, António
dc.contributor.authorAlbuquerque, Patricia
dc.date.accessioned2026-01-05T09:42:25Z
dc.date.available2026-01-05T09:42:25Z
dc.date.issued2025-11-27
dc.description.abstractEndophytic fungi are recognized for their capacity to produce bioactive metabolites, yet their metabolic output is strongly modulated by preservation strategies. This study evaluated the influence of three long-term preservation methods—PDA slants with mineral oil (PB), Castellani’s method (PC), and filter paper with mineral oil (PF)—on the production of phenolic compounds and antioxidant activity by the Amazonian endophytes Cophinforma mamane and Aspergillus niger. Post-reactivation morphological analyses revealed method-dependent changes in colony pigmentation, texture, and sporulation intensity. PB preservation induced the most vigorous growth and enhanced sporulation in A. niger. For extracellular metabolites (AcOEt extracts), PB yielded the highest total phenolic content in A. niger (258.24 mg GAE/g), whereas PF and PC favored C. mamane (154.48 and 153.33 mg GAE/g). For intracellular ethanolic extracts, PB was most effective for C. mamane, producing 206.19 mg GAE/g for total phenols and the highest antioxidant activities (EC50 = 1.40 mg/mL and FRAP = 95.61 μmol TE/g). HPLC–DAD identified protocatechuic acid as the predominant compound, which was especially abundant in PC and PF-preserved A. niger (60.76 and 85.38 μg/mg, respectively). Multivariate correlations indicated syringic (r = 0.53) and p-coumaric (r = 0.63) acids as the main contributors to antioxidant potential. These findings demonstrate that preservation methods may positively or negatively modulate fungal biosynthetic capacity, underscoring the importance of species-specific preservation protocols for maintaining metabolic stability in culture collections.por
dc.identifier.citationPadrón-Antonio, Y.; Falcão, L.d.S.; de Souza, A.T.F.; de Andrade, C.P.; da Silva, P.V.M.; Barbosa, L.K.; Duvoisin, S., Jr.; Correia, A.C.; Jordão, A.M.; Albuquerque, P.M. Comparative Analysis of Secondary Metabolite Production by Cophinforma mamane and Aspergillus niger Preserved for Long-Term Methods. Processes 2025, 13, 3834.
dc.identifier.doi10.3390/pr13123834
dc.identifier.urihttp://hdl.handle.net/10400.19/9605
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectphenolic compounds
dc.subjectantioxidant activity
dc.subjectpreservation techniques
dc.subjectAmazonian biodiversit
dc.subjectsecondary metabolites
dc.subjectendophytic fungi
dc.titleComparative Analysis of Secondary Metabolite Production by Cophinforma mamane and Aspergillus niger Preserved for Long-Term Methodseng
dc.typedataset
dspace.entity.typePublication
oaire.citation.titleProcesses
oaire.citation.volume13
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCorreia
person.familyNameJordão
person.givenNameAna Cristina
person.givenNameAntónio
person.identifier.ciencia-id5214-400C-AC20
person.identifier.ciencia-id1212-F02A-B14D
person.identifier.orcid0000-0002-6283-1964
person.identifier.orcid0000-0003-1129-4633
person.identifier.scopus-author-id34770030700
relation.isAuthorOfPublication6083bdfd-0c17-40aa-af2c-cefb2ebf7ac3
relation.isAuthorOfPublication32c0ef5c-f36b-4810-b994-e14eea9fa04f
relation.isAuthorOfPublication.latestForDiscovery6083bdfd-0c17-40aa-af2c-cefb2ebf7ac3

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