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Advisor(s)
Abstract(s)
This work aimed at comparing different extracting conditions in order to enhance the extraction of phenolic compounds with
antioxidant activity from strawberry. For the laboratorial tests, the strawberries were triturated and samples of 5 g were
taken to perform extractions with methanol, for different times (15 and 60 min.) and using different volumes of the
extraction solvent (25 and 50 mL). For each of the combinations three successive extractions were made and in each extract
were quantified the total phenolic compounds (TPC), anthocyanins (ANT) and the antioxidant activity (AOA), all by
spectrophotometric techniques. The results indicated that the more adequate conditions for the extraction of phenolic
compounds and anthocyanins with antioxidant activity from strawberries corresponded to three successive extractions using
each lasting for 60 min and using 50 mL of methanol. These conditions potentiated the extraction of high amounts of
phenolic compounds with antioxidant activity. The TPC contents in the fresh strawberry varied from 2.2 to 3.2 mg GAE/g, the
ANT contents varied from 131 to 149 mg Mv3G/g and the AOA was evaluated in the range 12.6 to 23.9 µmol TE/g.
Description
Keywords
Antioxidant Activity Anthocyanins Bioactive phenols Extraction conditions
Pedagogical Context
Citation
Ferrão, A.C., Guiné, R.P.F., Gonçalves, F.J.A., Correia, P.M.R., & Gonçalves, J.C. (2017, December). Influence of extraction time and volume of extracting solution on the phenolic compounds and antioxidant activity of strawberry. In Abstract Book and Proceedings of International Congress on Engineering, Universidade da Beira Interior, Covilhã, Portugal, pp. 1-11.