ESAV - DZERV - Resumos de eventos científicos não indexados à WoS/Scopus
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Browsing ESAV - DZERV - Resumos de eventos científicos não indexados à WoS/Scopus by Author "Barros, M."
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- SalivaPrint: sheep saliva electrophoretic protein profile in a bioinformatics approachPublication . Esteves, E.; Fernandes, M.; Cruz, I.; Esteves, Fernando; Rosa, N.; Correia, M. J.; Vala, Helena; Barros, M.Introduction: Sheep saliva is a fluid with properties relevant for the evaluation of individual wellbeing and disease states. It can be used for individual monitoring and decision aiding in therapeutic intervention. The total protein profile of each subject (SalivaPrint) can be integrated with clinical and environmental data to stratify individuals and analyze their health status. This strategy can also support the discovery of novel biomarkers. This study applies a bioinformatics strategy in order to identify standard protein profiles in healthy sheep. Standard total protein profiles of sheep are compared to human profile. Methods: Saliva samples were collected using an adaptation of the collection method available in Rosa et al 2016.Total protein profile of sheep saliva samples was performed b capillary electrophoretic analysis using the Experion™ technology. An algorithm which is able to identify common molecular weights ranges in a group of electrophoretic profiles was used. The molecular weight ranges identified were then used to discern between sheep and human population. Common molecular weights present in sheep SalivaPrint were then used with OvisOme database to identify proteins. Using AgBase GORetriever tool it was possible to analyze and catalogue their biological process. Results: Sheep and Human SalivaPrints are different and it is possible to discern both species. The SalivaPrints of individuals from the same flock appear to have a higher degree of similarity than individuals of different flocks. Sheep SalivaPrints showed five commonly present molecular weight ranges 7,9-8,9; 8,9-9,8; 12,7-13,6; 17,4 18,4 and 27,928,8 kDa. In this ranges, 14 proteins were identified using OvisOme database. The most representative biological process found was the response to stress (imm ne system process) with the proteins: Cathelicidin-1, Beta 2 microglobulin, Cathelin, Kallirein and Chloride intracellular channel protein. Conclusion: It was possible to conclude that different species have different salivary protein profiles. In addition to this, the total protein profile seem to be highly conserved between flocks. By using SalivaPrint profiles with bioinformatic tools like OvisOme.