ESAV - DZERV - Resumos de eventos científicos não indexados à WoS/Scopus
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- New therapeutic approaches on chronic kidney diseasePublication . Nogueira, A.; Vala, Helena; Nóbrega, Carmen; Pires, C. A.; Colaço, B.; Oliveira, P. A.; Pires, M. J.Introduction: chronic kidney disease is a serious public health problem that affects millions of patients worldwide. So, the main research goals in the CKD patients are the research of new therapeutic approaches capable of slowing down the progression to end-stage renal disease. Objective: the aim of this work, was to evaluate the effects of chronic administration of chaetomellic acid A (CAA), which selectively blocks H-Ras farnesylation, on kidney chronic lesions in 5/6 nephrectomized Wistar rats, an animal model of chronic renal disease. Material and methods: sixty male Wistar rats (Rattus norvergicus) were housed under controlled conditions. After seven weeks of acclimatization, rats (weighing 359 to 402 g) were sham-operated (SO) or submitted to 5/6 nephrectomy (RMR). One week after surgery surviving animals (n=53) were distributed into four groups: SO: SO rats receiving no treatment (n=13); SO+CAA: SO rats receiving CAA treatment (n=13); RMR: RMR rats receiving no treatment (n=14); RMR+CAA: RMR rats receiving CAA treatment (n=13). CAA was intraperitoneally administered (0.23 µg/Kg) three times a week for six months. Six months after the surgical procedure, in the left kidney of each animal was evaluated the mean cortical and medullary echogenicity by two-dimensional ultrasonography. Then, the kidneys were removed, fixed in 10% neutral-buffered formalin and processed for routine histopathological diagnosis and chronic lesions were evaluated, by Hematoxylin-Eosin, reticulin and Masson trichrome methods, for severity. All experimental procedures followed the European (European Directive 2010/63/EU) and National (Decree-Law 113/2013) legislation on the protection of the animals used for scientific purposes. Results: the kidneys of the RMR animals treated with CAA showed a significantly decrease in the medullary echogenicity (p<0.05) compared with the RMR rats that received no treatment. Glomerulosclerosis and arteriolosclerosis scores were significantly lower (p<0.001) in the RMR+CAA group when compared with the RMR group. There were no significant differences in interstitial fibrosis, interstitial inflammation and tubular dilatation scores between the RMR+CAA and RMR groups.Conclusion: this data suggests that pharmacological inhibition of H-Ras proteins activation may be a future strategy in the prevention of end-stage renal disease.
- Hepatic histopathological lesions in acute controled haemorrhage followed by volume replacement with a crystalloid or colloid solutionPublication . Vala, Helena; Pina, R.; Cruz, R.; Venâncio, C.; Esteves, Fernando; Silva, A.; Mesquita, João; Ortiz, A. L.; Ferreira, D.Introduction: severe hemorrhage remains the major cause of morbidity and mortality in trauma victims or surgical intervened animals, despite of all the advances in the therapeutic approach. The resulting injuries, or even death, are due to the deficit in intravascular volume and subsequent development of hypovolaemic fluid state, leading to poor tissue perfusion and consequent decreased oxygen delivery to the tissues, often with compromise of organ function. Objective: the aim of this work was to evaluate the effect of different intravenous solutions used for volume replacement following acute controlled haemorrhage, one crystalloid (RL) and one colloid (HES130/0.4) in the integrity of the hepatic tissue in a pig animal model. Material and methods: hepatic samples were collected from animals submitted to passive arterial blood bleeding and reperfusion with a crystalloid (RL) (G1) and with a synthetic colloid (HES 130/0.4) (G2). Samples were also collected from animals that were not subjected to acute bleeding nor volume replacement (G3, control group). All procedures were carried out under personal and project licenses approved by the Ethical Committee of the national regulatory office. Samples were collected and fixed in 10% neutral-buffered formalin, for a maximum of 24hours, embedded in paraffin wax and 3µm sections were stained for routine histopathology with haematoxylin and eosin. Results: the histopathological assessment revealed no statistically significant differences between the three groups. However, some lesions were more often expressed in some groups. More severe hepatocellular hydropic degeneration and hepatocellular steatosis was seen in G1, which is the only group in which haemorrhage was observed and within which oedema was not present. Hyperaemia was only observed in G2 and G3. Necrosis was not present in any of the groups. Conclusion: hepatic histopathological lesions following controlled bleeding and intravenous volume replacement with RL or HES130/0.4 were subtle. However, more pronounced hydropic degeneration and hepatocellular steatosis was seen in G1 (RL), which suggests that HES130/04 may be associated with better hepatic perfusion when used for intravenous volume replacement when compared to RL.
- Chronic splenic lesions in surviving pigs from outbreaks of gastric disordersPublication . Vala, Helena; Carreira, Patrícia; Esteves, Fernando; Santos, Carla; Cruz, Rita; Mesquita, João R.; Nóbrega, Carmen; Resendes, A.; Ségalez, J.The gastric problems in pigs acquired the new expression as intensive practices currently adoptes. The authors document, using macro and microscopic figures, a case of high mortality rate in a sow breeding stock in an indoor farm, aiming to discuss the best resolution to adopt. A breeding stock with 500 sows and high animal welfare standards, presented outbreaks of sudden death, in 2014 and 2016, that led to nutritional management changes. Several sows were necropsied and samples fixed in 10% neutral-buffered formalin and processed for routine histopathological diagnosis. Macroscopic exam, realized during outbreaks, revealed severe gastric ulcers and/or torsions. During the present year, cases of death, due to different etiologies, were necropsied and also showed splenic atrophy, irregularities and thickening at the capsule level. Microscopic exam revealed hypertrophy of smooth muscle of the capsule and splenic trabeculae, with presence of siderocalcary and hemorrhagic nodules. The authors suggest the diagnosis of postprandial gastric dilatation in animals surviving the outbreaks reported, with severe spleen repercussions that are still observed on the farm.
- Walking against cancer: the impact of long-term exercise training on mammary tumorsPublication . Rocha, A. I. Faustino; Oliveira, P. A.; Gama, A.; Vala, Helena; Esteves, Fernando; Ferreira, R.; Ginja, M.Breast cancer is one of the most frequent cancers among women worldwide. The present work evaluated the effects of long-term exercise training on the development of chemically-induced mammary cancer. Materials and Methods: Procedures followed the European legislation and were approved by the Portuguese Ethics Committee (DGAV, approval nº008961). Thirty 7-week-old female Sprague-Dawley rats were intraperitoneally injected with the carcinogen N-methyl-N-nitrosourea (MNU) (50mg/Kg). After this, they were randomly divided into two experimental groups: sedentary (n=15) and exercised (n=15). Exercised animals were trained on a treadmill for 35 weeks. At the end of the study, the mammary tumors were collected, fixated in formalin and histologically evaluated. The expression of estrogen receptor (ER)α was assessed by immunohistochemistry. Results: An incidence of 100% was observed. A total of 51 mammary tumors was developed: 28 in sedentary group and 23 in exercised one (p>0.05). At histopathological analysis, it was observed that each mammary tumor exhibited more than one histological pattern. A total of 121 mammary lesions was counted: 71 in sedentary group and 50 in exercised group (p=0.056). The number of malignant lesions was higher in sedentary group when compared with the exercised one (39 versus 21) (p=0.020). All mammary lesions exhibited nuclear immunostaining for ERα; the immunoexpression was higher in exercised group when compared with the sedentary one (p<0.05). Conclusions: Long-term exercise training reduced the number and malignancy of mammary tumors. Additionally, it increased the ERα immunoexpression that is an indicator of tumor differentiation and better response to hormone therapy.
- A case of giant cell tumourPublication . Vala, Helena; Andrade, Luís; Nóbrega, Carmen; Santos, Carla; Cruz, Rita; Mega, Cristina; Esteves, Fernando; Gomes, A.; Mesquita, JoãoMalignant fibrous histiocytoma refers to a group of soft tissue tumours that represents a diagnostic challenge which must be based in the predominant cellular type present. The authors describe an unusual case of tumour in which the presence of giant cells was the distinctive feature. A 7 year old Cocker Spaniel male dog was presented with a subcutaneous nodule in the labial commissure, measuring 1.5 cm in diameter. Sample was fixed in 10 % buffered formalin solution, embedded in paraffin wax and 3 μm thick sections were stained for routine histopathological diagnosis with Haematoxylin and Eosin. A standard Streptavidin-Biotin Complex technique for immunohistochemical evaluation was also performed. Macroscopic examination revealed dark brown, slightly lobed tissues, alternating with whitish tissues, both with moderate consistency on the cut surface. Microscopic examination revealed ulceration and necrosis of the epidermis, under which a high cellular population of undifferentiated cells was observed. These cells population exhibited oval, normo or eucromatic nucleus, with notorious nucleolus and moderate cytoplasm that, resembled fusiform fenotype, intermixed with blood vessels and multinucleated giant cells, as well as intense immunoreactivity for vimentin. The authors suggest the diagnosis of malignant fibrous histiocytoma of giant cell subtype, based on the multinucleated giant cells predominance and submit it to the discussion, particularly for histological grade assessment and prognosis, since there is still some discrepancy in the literature.
- SalivaPrint: sheep saliva electrophoretic protein profile in a bioinformatics approachPublication . Esteves, E.; Fernandes, M.; Cruz, I.; Esteves, Fernando; Rosa, N.; Correia, M. J.; Vala, Helena; Barros, M.Introduction: Sheep saliva is a fluid with properties relevant for the evaluation of individual wellbeing and disease states. It can be used for individual monitoring and decision aiding in therapeutic intervention. The total protein profile of each subject (SalivaPrint) can be integrated with clinical and environmental data to stratify individuals and analyze their health status. This strategy can also support the discovery of novel biomarkers. This study applies a bioinformatics strategy in order to identify standard protein profiles in healthy sheep. Standard total protein profiles of sheep are compared to human profile. Methods: Saliva samples were collected using an adaptation of the collection method available in Rosa et al 2016.Total protein profile of sheep saliva samples was performed b capillary electrophoretic analysis using the Experion™ technology. An algorithm which is able to identify common molecular weights ranges in a group of electrophoretic profiles was used. The molecular weight ranges identified were then used to discern between sheep and human population. Common molecular weights present in sheep SalivaPrint were then used with OvisOme database to identify proteins. Using AgBase GORetriever tool it was possible to analyze and catalogue their biological process. Results: Sheep and Human SalivaPrints are different and it is possible to discern both species. The SalivaPrints of individuals from the same flock appear to have a higher degree of similarity than individuals of different flocks. Sheep SalivaPrints showed five commonly present molecular weight ranges 7,9-8,9; 8,9-9,8; 12,7-13,6; 17,4 18,4 and 27,928,8 kDa. In this ranges, 14 proteins were identified using OvisOme database. The most representative biological process found was the response to stress (imm ne system process) with the proteins: Cathelicidin-1, Beta 2 microglobulin, Cathelin, Kallirein and Chloride intracellular channel protein. Conclusion: It was possible to conclude that different species have different salivary protein profiles. In addition to this, the total protein profile seem to be highly conserved between flocks. By using SalivaPrint profiles with bioinformatic tools like OvisOme.
- Soil application of acidified slurry as alternative to raw cattle-slurry injection to minimise gaseous emissions in Mediteranean conditionsPublication . Fangueiro, David; Pereira, José Luís; Surgy, Sónia; Vasconcelos, E.; Coutinho, João
- Impact of pig-slurry treatment and application techniques on ammonia and greenhouse gas emissions in a Mediterranean forage system.Publication . Carneiro, João; Pereira, José Luís; Surgy, Sónia; Soares, Diana; Monteiro, José; Trindade, Henrique; Coutinho, João; Fangueiro, David
- Characterisation of gaseous emissions from tunnel ventilated broiler buildings during winter season in PortugalPublication . Pereira, José Luís; Alves, Susana; Trindade, Henrique; Borges, João; Ferreira, Pedro
- Assessment of ammonia and carbon dioxide concentrations in a breeding hen building under Portuguese winterPublication . Pereira, José; Garcia, Carla; Ferreira, Sílvia; Pinheiro, Victor; Trindade, Henrique; Conde, André; Ferreira, Pedro