Percorrer por autor "Gallardo, Eugenia"
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- Miniaturized Solid Phase Extraction Techniques Applied to Natural ProductsPublication . Rosendo, Luana M.; Brinca, Ana Teresa; Pires, Bruno; Catarro, Gonçalo; Rosado, Tiago; Guiné, Raquel P. F.; Araújo, André R. T. S.; Anjos, Ofélia; Gallardo, EugeniaNatural products are increasingly becoming part of our daily lives through their use in industry, food, as therapeutic agents, etc. To evaluate their possible applications, it is essential to characterize them chemically to explore their potential. Different techniques may be used to characterize natural products, including microextraction techniques. These techniques have been gaining popularity due to the advantages associated with their low use of organic solvents and the small amount of sample used relative to more classical sample preparation techniques. Their application in the extraction of compounds from natural products is still scarce. This manuscript intends to review the most used solid-based miniaturized sample preparation techniques applied to determining compounds in natural products. The main applications of these methodologies will be discussed, with a particular focus on natural product analysis, as well as their advantages and disadvantages over traditionally used sample preparation techniques.
- Potential Applications of the Cytisus Shrub Species: Cytisus multiflorus, Cytisus scoparius, and Cytisus striatusPublication . Caramelo, Débora; Barroca, Celina; Guiné, Raquel; Gallardo, Eugenia; Anjos, Ofélia; Gominho, JorgeCytisus spp. is present throughout the Portuguese territory. Although most of these species are considered native in Portugal, at least one species has already been reported as invasive in other parts of the world. Different measures of control have been investigated, and the application of herbicides is the most commonly used. This review gathers information about the biochemical profile and analytical methods used for the evaluation of the potential bioactivities of three species of the genus Cytisus, better known as brooms, which were used in traditional medicine through the production of infusions and decoctions for the treatment of several health problems, mainly due to their high value of phenolic compounds. However, little research has been conducted on its biological activities as a potential antioxidant, anti-inflammatory, and antimicrobial agent. Furthermore, one species (Cytisus striatus) has not been subjected to extensive research in identifying chemical compounds and evaluating their potential bioactivities. This species (known as the Portuguese broom) has a great expression in one of the forest typologies with a considerable percentage in Portugal. This research work is essential to encourage a scientific and sustainable valorisation of Cytisus spp. (namely C. striatus), which will consequently contribute to forest cleaning and management to reduce the risk of wildfires.