Browsing by Author "Gomes, A."
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- A case of giant cell tumourPublication . Vala, Helena; Andrade, Luís; Nóbrega, Carmen; Santos, Carla; Cruz, Rita; Mega, Cristina; Esteves, Fernando; Gomes, A.; Mesquita, JoãoMalignant fibrous histiocytoma refers to a group of soft tissue tumours that represents a diagnostic challenge which must be based in the predominant cellular type present. The authors describe an unusual case of tumour in which the presence of giant cells was the distinctive feature. A 7 year old Cocker Spaniel male dog was presented with a subcutaneous nodule in the labial commissure, measuring 1.5 cm in diameter. Sample was fixed in 10 % buffered formalin solution, embedded in paraffin wax and 3 μm thick sections were stained for routine histopathological diagnosis with Haematoxylin and Eosin. A standard Streptavidin-Biotin Complex technique for immunohistochemical evaluation was also performed. Macroscopic examination revealed dark brown, slightly lobed tissues, alternating with whitish tissues, both with moderate consistency on the cut surface. Microscopic examination revealed ulceration and necrosis of the epidermis, under which a high cellular population of undifferentiated cells was observed. These cells population exhibited oval, normo or eucromatic nucleus, with notorious nucleolus and moderate cytoplasm that, resembled fusiform fenotype, intermixed with blood vessels and multinucleated giant cells, as well as intense immunoreactivity for vimentin. The authors suggest the diagnosis of malignant fibrous histiocytoma of giant cell subtype, based on the multinucleated giant cells predominance and submit it to the discussion, particularly for histological grade assessment and prognosis, since there is still some discrepancy in the literature.
- Dairy cattle-slurry treatment technologies: towards an integrating approach of crop production, animal feeding and environmental impactsPublication . Rodrigues, A.; Gomes, A.; Ferreira, L.; Trindade, H.; Fonseca, A.; Pereira, J.Dairy cattle farms have a well-known environmental impact that affects all ecological compartments: air, soil, water and biosphere [1]. Dairy cattle farming are a significant source of anthropogenic gases from enteric fermentation, manure storage and land application, mainly ammonia (NH3), nitric oxide (NO), nitrous oxide (N2O), carbon dioxide (CO2) and methane (CH4). The emission of such gases represents not only an environmental problem but also leads to energy and nitrogen (N) losses in ruminant production systems [2-5]. Several efforts are required on the development of new technologies and strategies that mitigate gaseous emissions, N losses and improve the efficiency of the energy and N cycles [6, 7]. In the Northwest of Portugal, dairy cattle production has a major impact on the economy, with strong repercussions at national scale. Therefore, our Ph.D. thesis project aims to: a) Study natural supplements as additives in the dairy cattle diet towards a decrease in GHG emissions from feeding operations; b) Compare commercial dairy cattle diets with and without additives on gaseous emissions from manure deposited in a simulated concrete floor; c) Assess the concentrations and emissions of NH3 and greenhouse gases from commercial dairy cattle facilities; d) Evaluate the effects of different additives on lowering gaseous emissions from dairy cattle excreta, using a laboratory system simulating a dairy house concrete floor.