Percorrer por autor "Melo, F."
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- Utilização de parâmetros quantitativos e semi-quantitativos para avaliação dos Ilhéus de Langerhans num modelo animal de Diabetes Mellitus tipo 2.Publication . Mega, C.; Vala, Helena; Teixeira de Lemos, E.; Fernandes, R.; Garrido, P.; Sereno, J.; Parada, B.; Melo, F.; Teixeira, F.; Reis, F.Introduction: Type 2 diabetes mellitus 2 (T2DM) results, amongst others, from insulin resistance and reduction of ß-cell secretion, produced by functional changes and cellular mass reduction. Aim: Chronological morphologic characterization of the endocrine pancreas in an animal model of T2DM, the Zucker Diabetic Fatty (ZDF) rats to elucidate the underlying mechanisms of disease evolution. Material and methods: The pancreas of diabetic, obese rats (ZDF Gmi fa/fa) and their littermates (ZDF Gmi +/+) were compared at 8, 20, 26 weeks of age. After routine stain haematoxylin-eosin. Quantitative and semi-quantitative parameters were applied for evaluation of morphological changes in the islets of Langerhans. Quantitative parameters included counting islet numbers and islet size measurement. Semi-quantitive parameters consisted in the evaluation of islet conformation and perimeter regularity. The inflammatory lesions, vascular congestion and β-cell vacuolization were also classified in a scale from 0 to III. Results: Obese, diabetic ZDF (fa/fa) rats presented, with increasing age, a reduction in islet numbers, progressive deformation of insular architecture, fibrosis and vascular congestion, connected with inflammatory changes and joined by a decrease in insular cellular mass. Conclusion: The coincidence of the progressive histopathologic changes with the metabolic profile obtained in our previous studies shows a decrease in the capacity of the endocrine pancreas to compensate insulin resistance and ß-cell dysfunction. These two conjunct facts make the ZDF fa/fa, rat an appropriate model for a variety of T2DM studies.