Percorrer por autor "Ortiz, Ana Liza"
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- Hepatocellular Early Apoptosis Associated with HES 130/0.4 Administration for Volume Replacement in Pigs After Severe BleedingPublication . Vala, Helena; Faustino-Rocha, Ana I.; Cruz, Rita; Venâncio, Carlos; Silva, Aura; Mesquita, João R.; Ortiz, Ana Liza; Ferreira, David A.Hydroxyethyl starch (HES) 130/0.4 is commonly used for volume replacement, yet its hepatic effects in the context of acute haemorrhage remain unclear. This study aimed to evaluate hepatic histopathological changes related to HES 130/0.4 administration when compared to Ringer’s lactate (RL) in healthy pigs subjected to acute bleeding under general anaesthesia. Eighteen pigs were randomised into three groups: RL (n = 6), HES 130/0.4 (n = 6), and a non-bleeding control (n = 6). Liver tissue was collected postmortem and analysed using haematoxylin–eosin staining, cytochrome c immunohistochemistry, the TUNEL assay, and M30 immunofluorescence. No statistically significant differences were observed in general histopathological changes, TUNEL, or cytochrome c expression (p > 0.050). However, the pigs that received HES 130/0.4 for volume replacement showed significantly higher intensity of the liver M30 immunostaining in the Q-score (p < 0.010), H-score (p < 0.010), and c indexc index (p < 0.050) when compared to animals that received Ringer’s lactate solution or animals in the control group. These findings suggest that HES 130/0.4 induces increased early hepatocellular apoptosis when compared to RL in this model, raising concerns about its hepatic safety profile under haemorrhagic conditions.
- Small Intestine mucosal injury and apoptosis following haemorrhage and volume replacement with different intravenous solutionsPublication . Ortiz, Ana Liza; Vala, Helena; Venâncio, Carlos; Garcia, Carla; Mesquita, João; Silva, Aura; Ferreira, DavidObjective: To characterize mucosal loss (ML) and apoptotic events in the small intestine after haemorrhage and volume replacement with a crystalloid, Lactated Ringers Solution (LR), and a colloid, HES130/0.4 solution, in a pig model. Methods: %ML and apoptotic events at the epithelium of the villi were evaluated in the small intestine from pigs that underwent total intravenous anaesthesia and passive controlled arterial bleeding, followed by volume replacement using LR (group1, n=9) and HES130/0.4 (group2, n=9). Apoptotic events were investigated by performing immunohistochemistry using Cytochrome c antibody and M30Cytodeath immunofluorescence assay. Results: %ML was significantly higher in group1 (P< 0.0001). Similar moderate to intense cytoplasmic immunoreactivity was seen in both groups for Cytochrome c antibody. The median percentage of apoptotic cells in the non-detached villi epithelium was 5.94% (1.46-31.47%) in group1 and 6.38% (0.36-47.48%) in group2. No significant differences were observed between groups. Conclusion: Results suggest that HES130/0.4 administration may more efficiently prevent the acute loss of intestinal mucosa, and, therefore, the integrity of the intestinal barrier. However, the indicators of intestinal apoptosis do not differ between HES 130/0.4 or RL administration.
- The influence of Ringer's lactate or HES 130/0.4 administration on the integrity of the small intestinal mucosa in a pig hemorrhagic shock model under general anesthesiaPublication . Ortiz, Ana Liza; Vala, Helena; Venâncio, Carlos; Mesquita, João; Silva, Aura; Gonzalo-Orden, Jose Manuel; Ferreira, DavidTo determine the effect of fluid resuscitation with 2 different physiological solutions, Ringer's lactate (RL) and hydroxyethyl starch (HES) 130/0.4, on histological lesions of the small intestinal mucosa in anesthetized pigs subjected to severe acute bleeding.