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- Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic SubtypesPublication . Sara Gomes-Gonçalves; Maria João Feiteiro; Guilherme Moreira; Rita Cruz; Fernando Esteves; Helena Vala; João R. Mesquita; Cruz, Rita; Esteves, Fernando; Vala Correia, Helena Maria
- Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic SubtypesPublication . Gomes-Gonçalves, Sara; Feiteiro, Maria João; Moreira, Guilherme; Cruz, Rita; Esteves, Fernando; Vala Correia, Helena Maria; Mesquita, João R.Research on endoparasites in pet dogs has been growing, but shepherd dogs have largely been overlooked. These dogs frequently share close proximity not only with sheep, which are reservoirs of zoonotic subtypes of Blastocystis, but also with their owners. This close contact increases the potential for shepherd dogs to act as intermediates in the transmission of Blastocystis. To clarify the role of these dogs as reservoirs for this parasite, this study investigated the presence of Blastocystis in shepherd dogs. Stool samples from Portuguese shepherd dogs were analyzed using SYBR-Green-based real-time PCR and melting curve analysis followed by targeted-amplicon NGS for mixed infections detection. Our results revealed a 60% occurrence of Blastocystis sp. in shepherd dog stools and frequent identification of zoonotic subtypes ST1–ST4 and ST14. Additionally, we observed mixed infections and subtype diversity within individual dogs, suggesting a potential role in cross-species transmission between livestock and humans.
- Strength and functional conditioning training: analyzing practitioners' perceptionsPublication . Rafael David; Azevedo, António; Mendes Ribeiro Eira, Paulo AlexandreIntroduction: Currently, both recreational participants and athletes are required to maintain fitness levels that enable them to endure the specific physical demands of their activities for longer periods. At the same time, they must be able to perform high-level technical movements during various sports activities, which are carefully planned to improve physical condition, well-being, and quality of life. Objective: This study aims to analyze participants' perceptions regarding the motivations that lead them to either engage in or avoid strength and functional conditioning training. Methodology: The study group consisted of 189 participants from strength and/or functional training facilities (boxes), spread across three municipalities in the district of Viseu. QMAD and IMAAD surveys were adapted for this application’s context, adding information to the four dimensions of the Exercise Motivation Inventory (EMI). Descriptive and inferential data analyses were performed using version 29 of SPSS software. Results: The motivations driving or limiting practice result from a complex interaction between personal, social, and environmental factors. Understanding the motivations or barriers associated with practice is essential for developing strategies that promote adherence and continuity, creating more stimulating learning environments, and better adapting training methodologies to participants' preferences and needs. Conclusions: Functional training is a fundamental approach to improving physical performance compared to traditional methods, as it confirms a substantial increase in physical capacity indices.