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Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain

2016-09Journal article Restricted access
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dc.contributor.authorMesquita, João R.
dc.contributor.authorOliveira, Danielle
dc.contributor.authorRivadulla, Enrique
dc.contributor.authorSilva, Joana Abreu
dc.contributor.authorVarela, Miguel F.
dc.contributor.authorRomalde, Jesús L.
dc.contributor.authorNascimento, Maria S. J.
dc.date.accessioned2017-01-26T13:20:44Z
dc.date.available2017-01-26T13:20:44Z
dc.date.issued2016-09
dc.description.abstractCoastal waters can become contaminated with both human waste from sewage treatment plants and runoff following manure application. Thus, shellfish produced close to land can bioaccumulate enteric viruses of human and animal origin, including zoonotic hepatitis E virus that infect both human and swine. The goal of this study was to evaluate the presence of HEV in shellfish from Galicia (NW Spain), a densely populated region with a strong tradition of swine farming, and one of the most important regions in the world for mussel production. We tested 81 mussel batches by RT-qPCR followed by conventional broad-spectrum nested RT-PCR and phylogenetic analysis. We have obtained 12 positive samples by RT-qPCR (14.81%) with HEV contamination levels ranging from 6.7 × 10(1) to 8.6 × 10(4) RNA copies/g digestive tissue. Phylogenetic analysis based on a 330 nt region of the ORF 1 showed that all sequenced isolates belonged to the zoonotic genotype 3 subgenotype e, being closely related to strains of human and swine origin. Results show that shellfish may be a potential route for HEV transmission to humans.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMesquita, J. R., Oliveira, D., Rivadulla, E., Silva, J. A., Varela, M. F., Romalde, J. L., & Nascimento, M. S. J. (2016). Hepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spain. Food Microbiology, 58, 13-15. doi:10.1016/j.fm.2016.03.009.pt_PT
dc.identifier.doi10.1016/j.fm.2016.03.009pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/4106
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.fm.2016.03.009pt_PT
dc.subjectDetectionpt_PT
dc.subjectHepatitis E viruspt_PT
dc.subjectShellfishpt_PT
dc.titleHepatitis E virus genotype 3 in mussels (Mytilus galloprovinciallis), Spainpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15pt_PT
oaire.citation.startPage13-5pt_PT
oaire.citation.titleFood Microbiologypt_PT
oaire.citation.volume58pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

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