Publication
Hepatocellular Early Apoptosis Associated with HES 130/0.4 Administration for Volume Replacement in Pigs After Severe Bleeding
| dc.contributor.author | Vala, Helena | en_US |
| dc.contributor.author | Faustino-Rocha, Ana I. | en_US |
| dc.contributor.author | Cruz, Rita | en_US |
| dc.contributor.author | Venâncio, Carlos | en_US |
| dc.contributor.author | Silva, Aura | en_US |
| dc.contributor.author | Mesquita, João R. | en_US |
| dc.contributor.author | Ortiz, Ana Liza | en_US |
| dc.contributor.author | Ferreira, David A. | en_US |
| dc.date.accessioned | 2025-12-22T14:37:01Z | |
| dc.date.available | 2025-12-22T14:37:01Z | |
| dc.date.issued | 2025-08-22 | en_US |
| dc.date.updated | 2025-12-22T12:31:49Z | |
| dc.description.abstract | Hydroxyethyl starch (HES) 130/0.4 is commonly used for volume replacement, yet its hepatic effects in the context of acute haemorrhage remain unclear. This study aimed to evaluate hepatic histopathological changes related to HES 130/0.4 administration when compared to Ringer’s lactate (RL) in healthy pigs subjected to acute bleeding under general anaesthesia. Eighteen pigs were randomised into three groups: RL (n = 6), HES 130/0.4 (n = 6), and a non-bleeding control (n = 6). Liver tissue was collected postmortem and analysed using haematoxylin–eosin staining, cytochrome c immunohistochemistry, the TUNEL assay, and M30 immunofluorescence. No statistically significant differences were observed in general histopathological changes, TUNEL, or cytochrome c expression (p > 0.050). However, the pigs that received HES 130/0.4 for volume replacement showed significantly higher intensity of the liver M30 immunostaining in the Q-score (p < 0.010), H-score (p < 0.010), and c indexc index (p < 0.050) when compared to animals that received Ringer’s lactate solution or animals in the control group. These findings suggest that HES 130/0.4 induces increased early hepatocellular apoptosis when compared to RL in this model, raising concerns about its hepatic safety profile under haemorrhagic conditions. | por |
| dc.description.version | N/A | |
| dc.identifier.doi | 10.3390/vetsci12090787 | en_US |
| dc.identifier.issn | 2306-7381 | en_US |
| dc.identifier.slug | cv-prod-4630383 | |
| dc.identifier.uri | http://hdl.handle.net/10400.19/9595 | |
| dc.language.iso | N/A | por |
| dc.subject | apoptosis | |
| dc.subject | hemorrhage | |
| dc.subject | HES 130/0.4 | |
| dc.subject | histopathology | |
| dc.subject | liver | |
| dc.title | Hepatocellular Early Apoptosis Associated with HES 130/0.4 Administration for Volume Replacement in Pigs After Severe Bleeding | en_US |
| dc.type | research article | en_US |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 9 | en_US |
| oaire.citation.startPage | 787 | |
| oaire.citation.title | Veterinary Sciences | en_US |
| oaire.citation.volume | 12 | en_US |
| rcaap.cv.cienciaid | 7A1E-E85E-FFA4 | Helena Maria Vala Correia | |
| rcaap.rights | openAccess | en_US |