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Routine histological technique applied to a biological model for environmental studies

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dc.contributor.authorVala, Helena
dc.contributor.authorRodriguez-Seijo, A.
dc.contributor.authorLourenço, J.
dc.contributor.authorGarcia, C.
dc.contributor.authorFrancisco, A.
dc.contributor.authorRocha-Santos, T.
dc.contributor.authorCosta, J.
dc.contributor.authorPereira, R.
dc.date.accessioned2016-06-06T08:29:20Z
dc.date.available2016-06-06T08:29:20Z
dc.date.issued2016
dc.description.abstractIntroduction and aim Earthworms are an important test organisms used in several standard ecotoxicological tests (e.g. ISO, 2008, 2012; OECD, 2004, 1094) and they are also model organisms to test soil structure and permeability, as well as for important soil services as the degradation organic matter. Although histopathological changes have been demonstrated to be good biomarkers to assess the exposure of these animals to different physical and chemical stress agents, studies with clear and high quality images describing normal tissue conditions are scarce in the literature, so the aim of this work was to better characterize this biological model. Material and Methods Eight adult earthworms exposed to an artificial standard soil (OECD, 2004) for 28 days, were extracted and placed in a plastic box to depurate their gut content, fixed in 10% neutral-buffered formalin and processed for routine histopathological diagnosis. Results and discussion Satisfactory histological sections were obtained. Some difficulties were faced related with microtome sectioning, resulting in artefacts, namely lines across sections produced by a nick in the cutting edge of the microtome knife cutting tear, motivated by the presence of sand and other solid particles that persisted in the gut of earthworms. Nevertheless, it was possible to obtain representative figures from different earthworm sections. Conclusion Routine histological technique was effective for obtaining satisfactory histological sections and the knowledge of the histology of earthworms could be very useful for future application in environmental studies, using this biological model.pt_PT
dc.identifier.citationVala H, Rodriguez-Seijo A, Lourenço J, Garcia C, Francisco A, Rocha-Santos T, Costa J, Pereira R (2016). Routine histological technique applied to a biological model for environmental studies. Abstracts in Conference Proceedings of the XXI Meeting of the Portuguese Society of Animal Pathology. Beyond Routine Diagnosis: 19-20pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/3200
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCITAB; FCT (UID/AGR/04033/2013 e POCI-01-0145-FEDER-006958). CI&DETS, FCTpt_PT
dc.subjectEarthwormspt_PT
dc.subjectecotoxicological testspt_PT
dc.subjectbiological model for environmental studiespt_PT
dc.subjectRoutine histological techniquept_PT
dc.titleRoutine histological technique applied to a biological model for environmental studiespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.endPage20pt_PT
oaire.citation.startPage19pt_PT
oaire.citation.titleConference Proceedings of the XXI Meeting of the Portuguese Society of Animal Pathology. Beyond Routine Diagnosispt_PT
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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