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Unraveling Sarcocystis miescheriana and Sarcocystis suihominis infections in wild boar

2015-09Journal article Restricted access
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dc.contributor.authorCoelho, Catarina
dc.contributor.authorGomes, Jacinto
dc.contributor.authorInácio, João
dc.contributor.authorAmaro, Ana
dc.contributor.authorMesquita, João Rodrigo
dc.contributor.authorPires, Isabel
dc.contributor.authorLopes, Ana Patrícia
dc.contributor.authorVieira-Pinto, Madalena
dc.date.accessioned2017-01-03T16:48:01Z
dc.date.available2017-01-03T16:48:01Z
dc.date.issued2015-09
dc.description.abstractSarcocystis species are worldwide spread cyst-forming protozoa that can infect wild boar but little is known about the prevalence of these parasites. In this study we assessed the prevalence of Sarcocystis spp. infections in wild boars from northeastern Portugal, for which novel PCR testing assays targeting Sarcocystis genus, S. miescheriana and S. suihominis were implemented, and risk factors potentially associated with these infections were evaluated. Samples from muscle tissue, namely diaphragm (n = 102), oesophagus (n = 96) and heart (n = 101), were collected from a total of 103 wild boar hunted between October 2011 and February 2012. Diaphragm muscle was used for the PCR detection of Sarcocystis nucleic acids since a higher proportion of samples showed the presence of cysts during histological examination. PCR assay targeting Sarcocystis genus yielded a 73.8% infection rate, which indicate a high level of exposure to these protozoan parasites among wild boars. These samples showed to be positive with the S. miescheriana-specific PCR assay and no sample was positive with the S. suihominis-specific assay, suggesting that a single species infecting wild boar is circulating in Portugal. These results were confirmed by the partial sequencing of the 18S rRNA gene amplified from selected samples from different geographic regions. Adults, young adults and female wild boars were found to be more likely infected. Hunters have an important role in the life cycle of S. miescheriana since potentially infected viscera and carcasses can be left behind promoting the protozoan dissemination to the scavenging final hosts. If hunting dogs bite and ingest infected meat they can perpetuate the life cycle of Sarcocystis spp. spreading oocysts or sporocysts in the environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCoelho, C., Gomes, J., Inácio, J., Amaro, A., Mesquita, J.R., Pires, I.,...Vieira-Pinto, M. (2015). Unraveling Sarcocystis miescheriana and Sarcocystis suihominis infections in wild boar. "Veterinary Parasitology", 212(3-4), 100-104. http://dx.doi.org/10.1016/j.vetpar.2015.08.015pt_PT
dc.identifier.doi10.1016/j.vetpar.2015.08.015pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/3594
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0304401715003982pt_PT
dc.subjectSarcocystis prevalencept_PT
dc.subjectRisk factorspt_PT
dc.subjectWild boarpt_PT
dc.subjectMolecular detectionpt_PT
dc.subjectSarcocystis miescherianapt_PT
dc.subjectSarcocystis suihominispt_PT
dc.titleUnraveling Sarcocystis miescheriana and Sarcocystis suihominis infections in wild boarpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage104pt_PT
oaire.citation.startPage100pt_PT
oaire.citation.titleVeterinary Parasitologypt_PT
oaire.citation.volume212pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT

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