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Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypovirus

2020Journal article Open access
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dc.contributor.authorOuni, Omar Abdelaziz
dc.contributor.authorJorge, Lurdes
dc.contributor.authorMoura, Luísa
dc.contributor.authorCoelho, Valentim
dc.contributor.authorGouveia, Eugénia
dc.date.accessioned2021-02-23T17:18:23Z
dc.date.available2021-02-23T17:18:23Z
dc.date.issued2020
dc.date.updated2021-02-23T16:44:15Z
dc.description.abstractIntroduction: Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (so-called cankers) on the bark of stems and branches of susceptible host trees. Cryphonectria hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence) and alters the fungus morphology in culture (pigmentation and sporulation capacity). By these characteristics, the strains with hypovirus CHV1 are used in Europe as a biological control agent of chestnut blight. Objectives: The aim of this work is to understand the effect of hypovirus on fungi virulence by comparing the production of some lignin-degrading enzymes and the metabolic profiles of some isogenic virulent and hypovirulent (converted and original) strains. Methods: The virulence of each strain was evaluated by mycelial inoculation on apple fruits (cv. Golden Delicious) and on detached, one year old, chestnut branches. To detect the activity of ligninolytic enzymes (laccases, peroxidases and cellulases), various substrates and indicator compounds were used. The metabolic profile of C. parasitica was evaluated by the Biolog FF system using 95 different carbon sources. Results: Virulent strains were found to cause more significant necrotic lesions in chestnut branches (p<0.05) and to produce larger lignin-degrading enzymes. The use of Biolog FF MicroPlates indicated that the use of 95 carbon sources five isolates of C. parasitica were significantly different (p <0.001), when the substrates were grouped into six types of chemical compounds. The highest AWCD values were obtained for carbohydrates, carboxylic acids and polymers, and the lowest values for amines/amides, amino acids and miscellaneous. Conclusions: Virulence evaluation of C. parasitica strains is important to study the hypovirulence processes mediated by the hypovirus CHV1. The detached branches of chestnut were, in our study, more suitable than apple fruits test in discriminating hypovirulent from virulent strains of C. parasitica. Virulent strains showed higher activity of acid-tannic inducible laccase (Lac3) and other lignin-degrading enzymes (LiP, MnP, and cellulase) when compared with hypovirulent ones. The results of the metabolic profiles studies may lead to new perspectives for understanding the biological process used by the hypovirus therefore, this may suggest a method for discriminating hypovirulent strains and study ecology and field fitness in this fungal strains.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOuni, O. A., Jorge, L., Moura, L., Coelho, V., & Gouveia, E. (2020). Virulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains converted by CHV1 hypovirus. Millenium, 2(13), 11-21. DOI: 10.29352/mill0213.01.00285pt_PT
dc.identifier.doi10.29352/mill0213.01.00285pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/6619
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Viseupt_PT
dc.relation.publisherversionhttps://revistas.rcaap.pt/millenium/article/view/19125pt_PT
dc.subjectCryphonectria parasiticapt_PT
dc.subjectvirulencept_PT
dc.subjectmetabolic profilept_PT
dc.subjectBiolog FF MicroPlatespt_PT
dc.subjectligninolytic enzymespt_PT
dc.subjectCryphonectria hypovirus 1 (CHV1)pt_PT
dc.titleVirulence, ligninolytic enzymes and metabolic profile of Cryphonectria parasitica virulent and hypovirulent strains Converted by CHV1 hypoviruspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceViseupt_PT
oaire.citation.endPage21pt_PT
oaire.citation.issue13pt_PT
oaire.citation.startPage11pt_PT
oaire.citation.titleMilleniumpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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RE - Série 2, n.º 13: (2020)