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Chemical characterization of pine bark (Pinus pinaster Aiton subsp. atlantica), antioxidant properties and phenolic profile of its extracts

2019Journal article Open access
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dc.contributor.authorVieito, Catarina
dc.contributor.authorPires, Preciosa
dc.contributor.authorFernandes, Élia
dc.contributor.authorVelho, Manuela
dc.date.accessioned2019-06-11T11:05:18Z
dc.date.accessioned2019-06-11T11:05:20Z
dc.date.available2019-06-11T11:05:18Z
dc.date.available2019-06-11T11:05:20Z
dc.date.issued2019
dc.description.abstractIntroduction: Pine bark is an agroindustrial residue from the timber industry and represents a source of phenolic compounds. These compounds have several beneficial properties being antioxidants, antimicrobial, anti-inflammatory, cardiovascular, among others. Objetives: The aim of this work was to study the chemical composition of the bark from Pinus pinaster Aiton subsp. atlantica and the phenolic profile of its aqueous, ethanolic and hydroethanolic extracts. Methods: The moisture content, ash, protein, crude fat and carbohydrates were analysed. The bark was extracted with water, ethanol or a mixture of both in a Soxhlet apparatus and the extraction yield, total phenolic content (TPC), antioxidant activity and phenolic profile by RP-HPLC-UV, in the extracts with higher TPC, were determined. Results: The results obtained for chemical composition were: 63.43 of carbohydrates, 2.81 of crude fat, 1.60 of proteins and 1.75 of ash, calculated in % w/w of dry bark. The extraction yield was greater for the ethanolic and the hydroethanolic extracts (17.08 and 17.55% w/w dry bark, respectively). The TPC and antioxidant activity were higher in the hydroethanolic extract (73.48 mg GAE/g and 108.74 mg AAE/g dry bark, respectively). Regarding the phenolic profile of the hydroethanolic extract, gallic acid, taxifolin, ferulic acid and quercetin were identified at 280 nm, and catechin was identified in the ethanolic extract at 320 nm. Conclusion: P. pinaster Aiton subsp. atlantica bark is mainly constituted by carbohydrates and it is rich in hydroethanolic and ethanolic extractives, being that these have high antioxidant activity. The ethanolic extract presents higher catechin amount when compared to the hydroethanolic extract.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.29352/mill0208.07.00197pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/5529
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Viseupt_PT
dc.relation.publisherversionhttps://revistas.rcaap.pt/millenium/article/view/14587pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectpine barkpt_PT
dc.subjectantioxidant propertiespt_PT
dc.subjectphenolic compoundspt_PT
dc.subjectRP-HPLC-UVpt_PT
dc.titleChemical characterization of pine bark (Pinus pinaster Aiton subsp. atlantica), antioxidant properties and phenolic profile of its extractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceViseupt_PT
oaire.citation.endPage87pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage79pt_PT
oaire.citation.titleMilleniumpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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RE - Série 2, n.º 08: (2019)