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Serra da Estrela cheese’s free amino acids profiles by UPLC-DAD-MS/MS and their application for cheese origin assessment

2019-10Journal article Restricted access
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dc.contributor.authorReis Lima, M.J.
dc.contributor.authorSantos, Andréia O.
dc.contributor.authorFalcão, Soraia I.
dc.contributor.authorFontes, Luísa
dc.contributor.authorLemos, Edite Teixeira de
dc.contributor.authorVilas-Boas, Miguel
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorPeres, António
dc.date.accessioned2019-11-12T15:19:40Z
dc.date.available2019-11-12T15:19:40Z
dc.date.issued2019-10
dc.description.abstractSerra da Estrela cheese is a high-value Portuguese Protected Designation of Origin cheese, produced with raw ewe milk. Thus, information regarding its composition is of utmost relevance for both consumers and certified producers. In this work, the chromatographic profiles of free amino acids in cheeses (45 days of maturation, 6 producers located in 5 municipalities and produced from November 2017 to March 2018) were established by UPLC-DAD-MS/MS. The proposed method allowed detecting 19 free amino acids and cystine with overall limits of detection and quantification lower than 44 μmol/L (1.4 mg/100 g cheese, wet matter) and then 134 μmol/L (4.2 mg/100 g cheese, wet matter), respectively. In all cheeses, 17 free amino acids were quantified including 8 essential amino acids (histidine, leucine-isoleucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine) and 9 non-essential amino acids (arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, proline, serine and tyrosine). The amounts of the free amino acids, essential free amino acids, branched chain free amino acids (leucine, isoleucine and valine) plus the free amino acids ratios (mg/g protein) were further used to identify the producer of Serra da Estrela cheeses. Linear discriminant analysis coupled with the simulated annealing variable selection algorithm was used allowing the correct classification of 96% and 90±8% of the samples, for leave-one-out and repeated K-fold cross-validation procedures, respectively. The satisfactory predictive performance pointed out the possibility of using cheeses’ amino acids profiles as origin biomarkers for authenticity control, warranting the correctness identification of the cheese producer/brand, which is quite relevant for ensuring the consumer confidence and satisfaction when purchasing this high-value dairy food.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationLima, M. J. R., Santos, A. O., Falcão, S., Fontes, L., Lemos, E.T., Vilas-Boas, M., … Peres, A. M. (2019). Serra da Estrela cheese’s free amino acids profiles by UPLC-DAD-MS/MS and their application for cheese origin assessment. Food Research International, (September), 108729. doi: https://doi.org/10.1016/j.foodres.2019.108729pt_PT
dc.identifier.doi10.1016/j.foodres.2019.108729pt_PT
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10400.19/5796
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0963996919306155?via%3Dihubpt_PT
dc.subjectSerra da Estrela cheesept_PT
dc.subjectAmino acids profilespt_PT
dc.subjectUPLC-DAD-MS/MSpt_PT
dc.subjectOrigin assessmentpt_PT
dc.subjectChemometricspt_PT
dc.titleSerra da Estrela cheese’s free amino acids profiles by UPLC-DAD-MS/MS and their application for cheese origin assessmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFood Research Internationalpt_PT
person.familyNameFalcão
person.familyNameTeixeira-Lemos
person.familyNameVilas-Boas
person.familyNamePeres
person.givenNameSoraia I.
person.givenNameEdite
person.givenNameMiguel
person.givenNameAntónio
person.identifier711432
person.identifier1395515
person.identifier1693134
person.identifier107333
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.ciencia-idF71E-A443-C8CB
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0003-3735-6951
person.identifier.orcid0000-0002-6346-8319
person.identifier.orcid0000-0002-8665-5280
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-5949-2013
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id14832459200
person.identifier.scopus-author-id22836614300
person.identifier.scopus-author-id6602648497
person.identifier.scopus-author-id7102331969
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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