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Advisor(s)
Abstract(s)
In this work were studied three red fruits (raspberry, gooseberry and blueberry) produced in organic mode, to evaluate the
variations in the content of phenolic compounds and antioxidant capacity along maturation. The phenols were extracted from
the fruits with two solvents (methanol and acetone) and were quantified by the Folin-Ciocalteu method. The antioxidant
activity was determined with two methods (HPPH and ABTS). Furthermore, HPLC was used to identify and quantify some
phenolic compounds present in the fruits analyzed. The results showed that the total phenolic compounds in all fruits decreased
along maturation, either in the methanol or acetone extracts (23 % and 20 % reduction, on average, for methanol and acetone
extracts, respectively), although in methanol extracts the levels of phenolic compounds were always higher (0.54 and 0.21 mg
GAE/g). The blueberry showed higher level of total phenolics in methanol extract (average 0.67 mg GAE/g), while in the
acetone extract it was gooseberry (average 0.31 mg GAE/g). At the end of maturation, all fruits studied had similar values of
antioxidant capacity as determined by DPPH method (0.52 mmol Trolox/g). For the ABTS method, blueberries showed higher
values of antioxidant activity (6.01 mmol Trolox/g against 3.01 and 2.66 mmol Trolox/g, for raspberry and gooseberry,
respectively). Furthermore, the HPLC analysis allowed to identify monomeric anthocyanins and phenolic acids in the three
fruits studied.
Description
Keywords
antioxidant activity blueberry gooseberry raspberry organic farming phenolic compounds
Citation
Guiné RPF, Soutinho SMA, Gonçalves FJ. (2014) Phenolic compounds and antioxidant activity in red fruits produced in organic farming. Croatian Journal of Food Science and Technololy, 6(1), 15-26.