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Structural characterization of polysaccharides isolated from grape stalks of Vitis vinifera L.

2012Journal article Open access
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dc.contributor.authorProzil, Sónia O.
dc.contributor.authorCosta, Elisabete V.
dc.contributor.authorEvtuguin, Dmitry V.
dc.contributor.authorCruz-Lopes, Luísa
dc.contributor.authorDomingues, M. Rosário M.
dc.date.accessioned2023-01-13T11:24:42Z
dc.date.available2023-01-13T11:24:42Z
dc.date.issued2012
dc.date.updated2023-01-12T12:33:05Z
dc.description.abstractThe main structural polysaccharides of grape stalks are cellulose, heteroxylan, and glucan. Cellulose contributes 30.3% of grape stalk matter and has an unusually high degree of the crystallinity (75.4%). Among hemicelluloses, xylan was the most abundant one, contributing ∼12% to the weight. The heteroxylan was isolated from the corresponding peracetic holocellulose by DMSO extraction followed by precipitation in ethanol. The Mw of heteroxylan (19.0kDa) was assessed by size exclusion chromatography (SEC) and the structure was inferred by methanolysis and methylation linkage analysis, as well as 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The heteroxylan is a partially acetylated (DS=0.49) glucuronoxylan possessing the main backbone composed by β-(1→4)-linked d-xylopyranosyl units ramified with α-(1→2)-linked 4-O-methyl-α-d-glucuronosyl residues (MeGlcpA) at a molar ratio 25:1. The isolated heteroxylan contained concomitant β-glucan (ca. 15%), whose structure was elucidated by methylation linkage analysis and by NMR spectroscopy. The results obtained revealed mixed β-(1→3; 1→4)-d-glucan with a molar ratio of β-(1→3)- to β-(1→4)-linked glucopyranosyl units of 1:2.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationProzil, S. O., Costa, E. V., Evtuguin, D. V., Cruz Lopes, L. P., & Domingues, M. R. M. (2012). Structural characterization of polysaccharides isolated from grape stalks of Vitis vinifera L. Carbohydrate Research, 356, 252–259. https://doi.org/10.1016/j.carres.2012.02.001pt_PT
dc.identifier.doi10.1016/j.carres.2012.02.001pt_PT
dc.identifier.eid2-s2.0-84862332115
dc.identifier.issn0008-6215
dc.identifier.slugcv-prod-1504282
dc.identifier.urihttp://hdl.handle.net/10400.19/7545
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectGrape stalkspt_PT
dc.subjectCellulosept_PT
dc.subjectHeteroxylanpt_PT
dc.subjectNMR spectroscopypt_PT
dc.subjectβ-Glucanpt_PT
dc.subjectESI-MSpt_PT
dc.titleStructural characterization of polysaccharides isolated from grape stalks of Vitis vinifera L.pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage259pt_PT
oaire.citation.startPage252pt_PT
oaire.citation.titleCarbohydrate Researchpt_PT
oaire.citation.volume328pt_PT
person.familyNameGonçalves Oliveira Valente da Cruz-Lopes
person.givenNameLuísa Paula
person.identifier.ciencia-id7916-0833-5B38
person.identifier.orcid0000-0001-6502-7202
rcaap.cv.cienciaid7916-0833-5B38 | Luísa Paula Gonçalves Oliveira Valente da Cruz-Lopes
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationfa1274f5-f7f8-4afd-8ed8-5f988d6c7226
relation.isAuthorOfPublication.latestForDiscoveryfa1274f5-f7f8-4afd-8ed8-5f988d6c7226

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