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Extraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansies

2019Journal article Open access
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dc.contributor.authorRamalhosa, Elsa
dc.contributor.authorFernandes, Luana
dc.contributor.authorPereira, José
dc.contributor.authorSaraiva, Jorge
dc.contributor.authorCasal, Susana
dc.date.accessioned2019-06-11T11:07:22Z
dc.date.available2019-06-11T11:07:22Z
dc.date.issued2019
dc.description.abstractIntroduction: Pansies (Viola×wittrockiana) are a rich source of natural antioxidants with beneficial effects on human health. Objetives: The aim of our study was to investigate solvents’ influence (water, methanol, water:acetone (6:4, v/v)) on the extraction of bioactive compounds and antioxidant activity of pansies extracts. Methods: The bioactive compounds analyzed were the following: flavonoids, hydrolysable tannins and monomeric anthocyanins, as well as total phenols by the total reducing capacity assay (TRC). The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power assays. A Principal Component Analysis (PCA) was performed to differentiate pansies extracts. Results: The solvents that yielded extracts with the highest contents of hydrolysable tannins and TRC were methanol and water:acetone (6:4, v/v). To extract the highest contents of monomeric anthocyanins, methanol should be used (5.93 mg Cy 3-glu/g flower, d.w), while for flavonoids, water:acetone (6:4, v/v) was the preferred yielding an extract with 115 mg QE/g flower d.w. Water turned out to be the least effective solvent, giving extracts with the lowest antioxidant activity. In addition, methanol or water:acetone extracts were clearly distinguished from aqueous ones through a PCA analysis. Conclusions: Our results show that the bioactive compounds and antioxidant activity of pansies’ extracts are affected by the solvent used.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.29352/mill0208.08.00205pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/5530
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Viseupt_PT
dc.relation.publisherversionhttps://revistas.rcaap.pt/millenium/article/view/14752pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectpansiespt_PT
dc.subjectsolventspt_PT
dc.subjectantioxidant activitypt_PT
dc.subjectbioactive compoundspt_PT
dc.titleExtraction solvents’ influence on the content of bioactive compounds and antioxidant activity of pansiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceViseupt_PT
oaire.citation.endPage98pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage89pt_PT
oaire.citation.titleMilleniumpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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RE - Série 2, n.º 08: (2019)