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Immunohistochemical detection of filaggrin in the skin of different species

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dc.contributor.authorBardagí, M.
dc.contributor.authorVala, Helena
dc.contributor.authorFondevila, D.
dc.contributor.authorFerrer, L.
dc.date.accessioned2013-02-05T16:54:22Z
dc.date.available2013-02-05T16:54:22Z
dc.date.issued2007
dc.description.abstractFilaggrin (FLG) is a key protein that facilitates terminal differentiation of the epidermis and formation of the skin barrier. Recent researches have demonstrated that, in humans, some variants of FLG are a major predisposing factor for atopic dermatitis. Furthermore, in immunohistochemical studies, FLG’s staining intensity was found significantly reduced in non-lesional and lesional atopic skin: FLG did not reach the horny layers and stained primarily granules in the stratum corneum. To the knowledge of the authors, the location of FLG in the skin of the domestic animals has not been investigated. Therefore, the aim of this research was to investigate, using immunohistochemistry, the expression of FLG in the skin of the dog, cat, horse, cow, pig and chicken. The long term purpose of this research line would be to investigate FLG expression in canine atopic skin. Formalin fixed, paraffin-emebedded sections were used in a ABC immunohistochemical technique. A monoclonal antibody against human FLG (Acrist Antibodies, Germany) was used as primary antibody. The results were very consistent in all species. FLG was detected clearly in the granular layer of the epidermis and in the outer root sheath of the hair follicles. In the chicken, all suprabasal layers were positive for FLG. The staining in all cases was cytoplasmic, being also the keratohyalin granules strongly stained. These results are very similar top those described in human beings and open the door to further researches regarding the FLG expression in animal skin diseasespor
dc.identifier.citationBardagí M, Vala H, Fondevila D, Ferrer L (2007). Immunohistochemical detection of filaggrin in the skin of different species. Proceedings of the 22nd Annual Congress of the ESVD-ECDV: 388por
dc.identifier.urihttp://hdl.handle.net/10400.19/1540
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectFilaggrinpor
dc.subjectskin barrierpor
dc.subjectdomestic animalspor
dc.subjectimmunohistochemistrypor
dc.titleImmunohistochemical detection of filaggrin in the skin of different speciespor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.startPage388por
oaire.citation.titleProceedings of the 22nd Annual Congress of the ESVD-ECDVpor
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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