ESAV - DZERV - Artigo em revista científica, não indexada ao WoS/Scopus
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- ABC series on diagnostic parasitology part 1: the Willis methodPublication . Mesquita, João R.; Esteves, Fernando; Santos, Carla; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Nóbrega, CármenDiagnosis of parasitic infections in animals is an interesting task that can be developed by veterinary nurses. To perform direct identification of parasites, particularly ova present in faeces, it is possible to use faecal smears, flotation and sedimentation tests. Faecal flotation is fast and inexpensive, and can be quickly implemented as a measure for infection control. Herewith we describe the Willis method, one of the most used flotation methods.
- ABC series on diagnostic parasitology part 2: the McMaster methodPublication . Nóbrega, Cármen; Santos, Carla; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Esteves, Fernando; Mesquita, João R.Classical coprological methods allow for inexpensive, quick and reliable detection of parasitic elements. However, the detection of these parasitic elements may be insufficient and quantification of the parasitic burden may be required. As such, faecal egg counts can play a crucial role in providing these extra data. Herewith we describe the McMaster method, one of the most used faecal egg count methods described.
- ABC series on diagnostic parasitology part 3: the Baermann techniquePublication . Mesquita, João R.; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Esteves, Fernando; Santos, Carla; Nóbrega, CármenDiagnosis of parasitic larval forms is typically associated with complex, costly methods. As such, simple, low-cost diagnostic tools for larvae identification are of the utmost importance in diagnostic parasitology in the veterinary clinic. Herewith we describe the Baermann technique, one of the most used larvae identification methods
- Adaptation of the ABCDE model from human medicine to communicate bad news to the owner of the oncologic patient in veterinary medicinePublication . Vala, Helena; Esteves, Fernando; Mega, Ana Cristina; Santos, Carla; Nóbrega, Carmen; Mesquita, JoãoSimilar to what occurs in Human Medicine, also in Veterinary Medicine, the prevalence of oncological diseases has significantly increased. The evolution of Veterinary Medicine, in last decades has brought changes in clinical paradigms, particularly concerning the relationship with the animal and also with the owner. More than any other specialty, members of the Veterinary Medical Team that work in the oncology field, are unavoidably forced to break bad news. This paper proposes the adaptation of the ABCDE model from Human Medicine to Veterinary Medicine. The adaptation of the ABCDE model for Veterinary Medicine improves communication with the owner and offers all the members of the Veterinary Medical Team better communication skills.
- Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expressionPublication . Costa, E.; Fernandes, J.; Ribeiro, S.; Garrido, P.; Rocha-Pereira, P.; Coimbra, S.; Catarino, C.; Reis, F.; Belo, L.; Bronze-da-Rocha, E.; Vala, Helena; Alves, R.; Santos-Silva, A.Our aim was to contribute to a better understanding of the pathophysiology of anemia in elderly, by studying how aging affects renal function, ironmetabolism, erythropoiesis and the inflammatory response, using an experimental animal model. The study was performed in male Wistar, a group of young rats with 2 months age and an old one with 18 months age. Old rats presented a significant higher urea, creatinine, interferon (INF)-gamma, ferritin and soluble transferrin receptor serum levels, as well as increased counts of reticulocytes and RDW. In addition, these rats showed significant lower erythropoietin (EPO) and iron serum levels. Concerning gene expression of iron regulatory proteins, old rats presented significantly higher mRNA levels of hepcidin (Hamp), transferrin (TF), transferrin receptor 2 (TfR2) and hemojuvelin (HJV); divalent metal transporter 1 (DMT1) mRNA levels were significantly higher in duodenal tissue; EPO gene expression was significantly higher in liver and lower in kidney, and the expression of the EPOR was significantly higher in both liver and kidney. Our results showed that aging is associated with impaired renal function, which could be in turn related with the inflammatory process and with a decline in EPO renal production. Moreover, we also propose that aging may be associatedwith INF-gamma-induced inflammation and with alterations upon iron regulatory proteins gene expression.
- Application of strategies to minimize the error in Pathological AnatomyPublication . Helena Vala; Fernando EstevesThe design of multi-professional teams, in the veterinary field allows, as in other sectors, the introduction and implementation of dynamics which don´t exhaust in the company itself. The main risks which compromise the quality and reliability of laboratory diagnosis, are related with incorrect methods of sampling, little information on the respective analysis request form or its incorrect fulfillment, failures in packing and sending samples to the laboratory, as well as the absence of indications to the correct orientation of the surgical specimens. In order to minimize the most detected errors, strategies are listed throughout the paper and, related to the correct orientation and identification of the sample, a proposal of identification is presented in this paper, which will be an important strategy for the minimization of analytical and post-analytical errors.
- Assessment of ammonia and carbon dioxide concentrations in a breeding hen building under Portuguese winterPublication . Pereira, José Luís; Ferreira, Sílvia; Garcia, Carla; Conde, André; Ferreira, Pedro; Pinheiro, Victor; Trindade, HenriqueExcessive ammonia (NH3) and carbon dioxide (CO2) in the housing of breeding hens can cause various negative effects on the health of hens and the welfare of the workers who care for them. The aim of this study was to evaluate the NH3 and CO2 concentrations in the first month of housing the breeding hens during Portuguese winter. The study was conducted on a commercial hen breeding farm located in central Portugal. One modern building equipped with climate control system, automatic feeding and drinking systems and minimum transitional tunnel ridge system was selected. New litter material made with rice hulls was used in the building, and the breeding sample comprised five months old 6864 female and 720 male birds housed in the building on 7 November 2016. The outdoor and indoor environmental conditions and indoor gas concentrations were measured continuously from 10 November to 30 November 2016. (NH3)and CO2 concentrations were measured with a photoacoustic field gas monitor and air samples collected through 4 sampling points located indoor, by a multipoint sampler. Results showed that the CO2 concentrations did not exceed 3000 ppm during the first month of housing the breeding hens and under winter environment. However, the NH3 concentrations exceed 20 ppm on most measurement days. For a good indoor air quality, the study suggests the use of mitigating measures for maintaining NH3 concentration below 10 ppm.
- Assessment of net nitrogen mineralisation from repeated applications of cattle-slurry and mineral fertiliser in a Mediterranean forage systemPublication . Pereira, J.; Coutinho, J.; Trindade, H.The aim of our study was to evaluate the impact of repeated applications of cattle-slurry and mineral fertiliser on net N mineralisation rates in a Mediterranean forage system, cropped with maize silage (spring-summer) followed by Italian ryegrass (autumn-winter) for silage. The field study was performed between May-1997 and May-2000 in a commercial dairy-cattle farm located in Northwest Portugal. Five treatments were employed: Control with no N fertiliser (T0); one application of cattle-slurry at maize sowing with 187 (T1) or 340 (T2) kg total N ha-1 year-1; two applications, 258 kg total N ha-1 at maize and 150 kg total N ha-1 at ryegrass sowing plus application of 190 kg total N ha-1 to maize and 50 kg total N ha-1 to ryegrass as mineral fertiliser, totalizing 648 kg total N ha-1 year-1 (T3), and; application of 240 kg total N ha-1 year-1 as mineral fertiliser to both crops like in T3 (T4). Net N mineralisation was measured in soil layer 0-30 cm in each treatment. Significant amounts of N were released (160-290 kg N ha-1 year-1) by mineralisation due cattle-slurry application to soil, with apparent net N mineralisation varying between 45 and 95% of the total N applied. The application of mineral fertiliser led to N immobilisation (-23%) of the total N applied. Data obtained in this study may be used in the management of the N fertilisation to improve manure-N efficiency.
- Blood Transfusions in Dogs and Cats: Blood Typing, Cross MatchingPublication . Branquinho, T.; Ortiz, V.; Vala, Helena; Ferreira, R.; Santos, C.; Oliveira, A. L.The blood transfusion (administration of blood) is a resource of veterinary medicine with several indications. Point-of-care blood-typing methods, including both typing cards and rapid gel agglutination, are readily available. Following blood typing, cross matching is performed on one or more donor units of appropriate blood type. Cross matching reduces the risk of transfusion reactions but does not completely eliminate the risk of other types of transfusion reactions in veterinary patients. All transfusion reactions should be appropriately documented and investigated. It is, therefore, important to have in-depth technical knowledge about concepts and procedures involved in clinical blood transfusion, so that the entire procedure can be performed in the most appropriate way and with a high success rate.
- Canine influenza: should we look out for a sneeze?Publication . Mesquita, João Rodrigo; Vala, Helena; Nóbrega, Carmennfluenza viruses are significant pathogens of mammals and birds but are rarely mentioned as important pathogens in dogs. Nevertheless, several viral episodes have occurred in past decade which are changing the comprehension of the influenza virus' role in dogs. Veterinary professionals can play an important role in preventing unreasonable fears that may arise among personnel and owners, and in improving understanding as to when and where influenza infection is possible.