Repository logo
 
Publication

Expression of estrogen receptors-α and β in chemically-induced mammary tumours

2015Conference object Restricted access
Simple item page
dc.contributor.authorFaustino-Rocha, A. I.
dc.contributor.authorGama, A.
dc.contributor.authorOliveira, P. A.
dc.contributor.authorAlvarado, A.
dc.contributor.authorVala, Helena
dc.contributor.authorFerreira, R.
dc.contributor.authorGinja, M.
dc.date.accessioned2016-02-17T16:54:53Z
dc.date.available2016-02-17T16:54:53Z
dc.date.issued2015
dc.description.abstractObjective: Estrogen receptors (ERs)-α and β are important for stimulating the growth and differentiation of human mammary tumours, respectively. This work aimed to evaluate the expression of ERs-α and β in rat chemically-induced mammary tumours. Methods: Procedures followed the European legislation (2010/63/EU). Fifteen seven-week-old female Sprague-Dawley rats received an intraperitoneal injection of N-methyl-N-nitrosourea (MNU) (50mg/Kg). At sacrifice, mammary tumours were fixed and histologically evaluated. Sections were incubated with ER-α and β primary antibodies and the percentage of immunopositive cells was determined. Results: During the experimental protocol four animals died, all survived animals developed mammary tumours: 30 benign, 2 preneoplastic and 39 malignant lesions. All histological patterns expressed ERs-α and β. The mean expression of ER-α (55.14% ± 13.26) was lower than the expression of ER-β (70.06% ± 18.51) (p<0.05). The expression of both receptors was not statistically different among lesions, however it was higher in preneoplastic lesions, followed by malignant and benign ones (p>0.05). Conclusion: All MNU-induced mammary tumours in rats expressed ERs-α and β, being a good model of ER-positive human mammary tumours. Once the expression of ER-β was higher, tumours’ differentiation was stimulated and its proliferation was inhibited.pt_PT
dc.identifier.citationFaustino-Rocha AI, Gama A, Oliveira PA, Alvarado A, Vala H, Ferreira R, Ginja M (2015). Expression of estrogen receptors-α and β in chemically-induced mammary tumours. Virchows Arch 467 (Supll I): S51pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/3076
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCI&DETS, FCT and QREN/FEDER (Ovislab ICT-2013-05-004-5314 ID-64757), (PTDC/DES/114122/2009)pt_PT
dc.subjectEstrogen receptors (ERs)-α and βpt_PT
dc.subjectrat chemically-induced mammary tumourspt_PT
dc.titleExpression of estrogen receptors-α and β in chemically-induced mammary tumourspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.titleVirchows Archpt_PT
oaire.citation.volume467pt_PT
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
59 Expression of estrogen receptors ECP 15.pdf
Size:
3.95 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESAV - DZERV - Documentos de congressos (comunicações, posters, actas)