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Immunohistochemical expression of apoptotic markers in renal tissue, in a model submitted to haemorrhage followed by volume replacement

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dc.contributor.authorVala, Helena
dc.contributor.authorCruz R, R.
dc.contributor.authorGarcia C, C.
dc.contributor.authorVenâncio C, C.
dc.contributor.authorMesquita J, J.
dc.contributor.authorSilva A, A.
dc.contributor.authorOrtiz AL, A. L.
dc.contributor.authorFerreira D, D.
dc.date.accessioned2016-02-17T16:49:41Z
dc.date.available2016-02-17T16:49:41Z
dc.date.issued2015
dc.description.abstractObjectives: To identify possible apoptotic events in renal tissue, using an animal model in which was simulated acute controlled haemorrhage, a frequent condition after a clinical situation resulting from a serious traumatic event, followed by volume replacement. Methods: 18 Large White pigs underwent total intravenous anaesthesia and randomly divided in three groups (n = 6). Group 1 and 2 were submitted to passive 25ml/kg arterial blood bleeding followed by volume replacement with LR in group1, and HES in group2, while group3 (control) was submitted to the same conditions, except bleeding and volume replacement. Renal tissue samples were processed for immunohistochemistry, using in situ TUNEL and Cytochrome c methods. The immunoreactivity levels were compared between groups using the Kruskal-Wallis test with Dunn’s post test. Results: Higher levels of apoptotic immunoreactivity were found for the HES group when compared to control and LR groups in convoluted tubules (p<0.01) and when compared to the control group in the descending limb of the loop of Henle and collecting duct. Conclusion: These findings are suggestive of a higher degree of insult in the tubular renal cells from animals that received HES 130/0.4.pt_PT
dc.description.sponsorshipFCT, project COMPETE: FCOMP-01-0124-FEDER-009525; IPV, CI&DETS, FCT and QREN/FEDER (Ovislab ICT-2013-05-004-5314 ID-64757)pt_PT
dc.identifier.citationVala H, Cruz R, Venâncio C, Garcia C, Mesquita J, Silva A, Ortiz AL, Ferreira D (2015). Immunohistochemical expression of apoptotic markers in renal tissue, in a model submitted to haemorrhage followed by volume replacement. Virchows Arch 467 (Supll I): S243pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/3074
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectImmunohistochemical expressionpt_PT
dc.subjectapoptotic markerspt_PT
dc.subjectrenal tissuept_PT
dc.subjectvolume replacementpt_PT
dc.subjectmodel submitted to haemorrhagept_PT
dc.titleImmunohistochemical expression of apoptotic markers in renal tissue, in a model submitted to haemorrhage followed by volume replacementpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.titleVirchows Archpt_PT
oaire.citation.volume467pt_PT
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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