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Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypes

2025-04-02Artigo de investigação Acesso aberto
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dc.contributor.authorGomes-Gonçalves, Sara
dc.contributor.authorFeiteiro, Maria João
dc.contributor.authorMoreira, Guilherme
dc.contributor.authorCruz, Rita
dc.contributor.authorEsteves, Fernando
dc.contributor.authorVala Correia, Helena Maria
dc.contributor.authorMesquita, João R.
dc.date.accessioned2026-02-06T13:57:43Z
dc.date.available2026-02-06T13:57:43Z
dc.date.issued2025-04-02en_US
dc.date.updated2026-01-31T18:12:47Z
dc.description.abstractResearch on endoparasites in pet dogs has been growing, but shepherd dogs have largely been overlooked. These dogs frequently share close proximity not only with sheep, which are reservoirs of zoonotic subtypes of Blastocystis, but also with their owners. This close contact increases the potential for shepherd dogs to act as intermediates in the transmission of Blastocystis. To clarify the role of these dogs as reservoirs for this parasite, this study investigated the presence of Blastocystis in shepherd dogs. Stool samples from Portuguese shepherd dogs were analyzed using SYBR-Green-based real-time PCR and melting curve analysis followed by targeted-amplicon NGS for mixed infections detection. Our results revealed a 60% occurrence of Blastocystis sp. in shepherd dog stools and frequent identification of zoonotic subtypes ST1–ST4 and ST14. Additionally, we observed mixed infections and subtype diversity within individual dogs, suggesting a potential role in cross-species transmission between livestock and humans.eng
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationGomes-Gonçalves, S.; Feiteiro, M.J.; Moreira, G.; Cruz, R.; Esteves, F.; Vala, H.; Mesquita, J.R. Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypes. Vet. Sci. 2025, 12, 325. https://doi.org/10.3390/ vetsci12040325
dc.identifier.doi10.3390/vetsci12040325en_US
dc.identifier.issn2306-7381en_US
dc.identifier.slugcv-prod-4702482
dc.identifier.urihttp://hdl.handle.net/10400.19/9662
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBlastocystis
dc.subjectshepherd dog
dc.subjectzoonotic subtypes
dc.subjectONT
dc.titleTargeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypesen_US
dc.typeresearch articleen_US
dspace.entity.typePublication
oaire.citation.issue4en_US
oaire.citation.startPage325
oaire.citation.titleVeterinary Sciencesen_US
oaire.citation.volume12en_US
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCruz
person.familyNameEsteves
person.familyNameVala Correia
person.givenNameRita
person.givenNameFernando
person.givenNameHelena Maria
person.identifier.ciencia-idA11E-DBFF-16E8
person.identifier.ciencia-id6E19-A3CC-897E
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0002-5767-7835
person.identifier.orcid0000-0003-0589-0746
person.identifier.orcid0000-0001-6829-4867
rcaap.cv.cienciaid6E19-A3CC-897E | Fernando Alexandre Almeida Esteves
rcaap.rightsopenAccessen_US
relation.isAuthorOfPublication8e60c18a-9c3f-4b0e-be9e-4378ebc1594e
relation.isAuthorOfPublication93d688a9-9716-444c-9d08-fdca593d630d
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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