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Small Intestine mucosal injury and apoptosis following haemorrhage and volume replacement with different intravenous solutions

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dc.contributor.authorOrtiz, Ana Liza
dc.contributor.authorVala, Helena
dc.contributor.authorVenâncio, Carlos
dc.contributor.authorGarcia, Carla
dc.contributor.authorMesquita, João
dc.contributor.authorSilva, Aura
dc.contributor.authorFerreira, David
dc.date.accessioned2016-02-17T16:51:33Z
dc.date.available2016-02-17T16:51:33Z
dc.date.issued2015
dc.description.abstractObjective: To characterize mucosal loss (ML) and apoptotic events in the small intestine after haemorrhage and volume replacement with a crystalloid, Lactated Ringers Solution (LR), and a colloid, HES130/0.4 solution, in a pig model. Methods: %ML and apoptotic events at the epithelium of the villi were evaluated in the small intestine from pigs that underwent total intravenous anaesthesia and passive controlled arterial bleeding, followed by volume replacement using LR (group1, n=9) and HES130/0.4 (group2, n=9). Apoptotic events were investigated by performing immunohistochemistry using Cytochrome c antibody and M30Cytodeath immunofluorescence assay. Results: %ML was significantly higher in group1 (P< 0.0001). Similar moderate to intense cytoplasmic immunoreactivity was seen in both groups for Cytochrome c antibody. The median percentage of apoptotic cells in the non-detached villi epithelium was 5.94% (1.46-31.47%) in group1 and 6.38% (0.36-47.48%) in group2. No significant differences were observed between groups. Conclusion: Results suggest that HES130/0.4 administration may more efficiently prevent the acute loss of intestinal mucosa, and, therefore, the integrity of the intestinal barrier. However, the indicators of intestinal apoptosis do not differ between HES 130/0.4 or RL administration.pt_PT
dc.identifier.citationAna Liza Ortiz, Helena Vala, Carlos Venâncio, Carla Garcia, João Mesquita, Aura Silva, David Ferreira (2015). Small Intestine mucosal injury and apoptosis following haemorrhage and volume replacement with different intravenous solutions. Virchows Arch 467 (Supll I): S243pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/3075
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationFCT, project COMPETE: FCOMP-01-0124-FEDER-009525; IPV, CI&DETS, FCT and QREN/FEDER (Ovislab ICT-2013-05-004-5314 ID-64757)pt_PT
dc.subjectSmall Intestine mucosal injurypt_PT
dc.subjectapoptosispt_PT
dc.subjecthaemorrhagept_PT
dc.subjectintravenous solutionspt_PT
dc.subjectpig modelpt_PT
dc.titleSmall Intestine mucosal injury and apoptosis following haemorrhage and volume replacement with different intravenous solutionspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.titleVirchows Archpt_PT
oaire.citation.volume467pt_PT
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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