First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR

Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.

Keywords

Borrelia burgdorferi sensu lato Wild boar PCR Fla gene Northern Portugal Lyme borreliosis Zoonosis One Health

URI

http://hdl.handle.net/10400.19/4290

Citation

Faria, A.S., Paiva-Cardoso, M. N., Nunes, M., Carreira, T., Vale-Cardoso, H., Veloso, O., ...Vieira, M. (2015). First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested- PCR. EcoHealth,12, 183-187. doi:10.1007/s10393-014-0973-4