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Abstract(s)
The distribution of keratins and the expression of cornified cell envelope proteins are well studied in epidermis and mucous membranes of human specie, laboratory species and, scarcely, in the dog. The aim of this study was to evaluate, by means of immunohistochemical techniques, the expression of these components in the nasal and labial mucocutaneous junctions from food-producing animals.
Samples from two mucocutaneous junctions were collected from goat, sheep, cow and pig, fixed in formalin 10% and embedded in paraffin.
For chacterization of keratins, two monoclonal antibodies (DAKO) were used. MNF116, labelling human keratins 5, 6, 8, 17 and 19 in non differentiated keratinocytes and simple epithelia and LP34, labelling human keratins 5, 6 and 18 in well-differentiated keratinocytes. The distribution of corneum envelope precursors was studied using two antibodies: monoclonal antibody anti-human involucrin (NOVOCASTRA), labelling keratinocytes of the upper stratum spinosum and stratum granulosum, and polyclonal antibody anti-human profilaggrin (ZYMED), labelling keratinocytes of the stratum granulosum.
With the MNF 116 antibody, the labial and nasal mucocutaneous junctions from goat, as well as labial from sheep and nasal from cow, revealed an intense reaction located at the suprabasale stratum. The labial mucocutaneous junction from pig was negative. With the LP34 antibody, were positive all suprabasale strata from both mucocutaneous junctions in ruminant species. In the pig, both mucous membranes were negatives.
With the anti-involucrin antibody, the nasal mucous membrane was positive in ruminants species, at the upper part of the stratum spinosum and in the stratum granulosum, but was negative in the pig. The labial mucous membrane was positive at the same layers in most species, except in the sheep. Both mucocutaneous junctions in all studied species were negative with the anti-profilagrin antibody.
It was possible to conclude that keratins were detected in nasal and labial mucous membranes from all studied species at supra-basale layers. In both mucous membranes from goat and cow, as well as in nasal mucous membrane from sheep and labial mucous membrane from pig, was also detected involucrin, at the upper part of the stratum spinosum. Both mucocutaneous junctions from all species didn’t revealed profilaggrin expression.
Description
Keywords
cornified cell envelope mucocutaneous junctions food-producing animals
Pedagogical Context
Citation
Vala H, Fondevila D, Ferrer L. Epithelial differentiation markers in mucucocutaneous junctions of different food-producing animals (2004). Proceedings of 12th Annual Meeting of the Portuguese Society of Animal Pathology jointly with 16th Annual Meeting of the Spanish Society of Veterinary Pathology: 220