| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 3.7 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Previously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized.
Although sharing the same type of raw material, most of their physicochemical, stability and maturity
properties differed. The present study aimed to characterize them at a microbiological level, and to infer
on possible relationships between the composts properties and the structure of their bacterial communities.
Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed
that the structure of the bacterial communities of these composts was distinct. The bacterial community
of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical
conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2,
contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic
(Halomonadaceae and Brevibacteriaceae) and metal resistant organisms (Brevibacteriaceae and
Bacillaceae) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and
Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of
MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population
were the parameters most correlated with the structure of the bacterial community of compost
MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly
found in composts.
Description
Keywords
Bacterial community Diversity DGGE profiling 454-pyrosequencing Multivariate analysis
Citation
Publisher
Elsevier