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Utilização de parâmetros quantitativos e semi-quantitativos para avaliação dos Ilhéus de Langerhans num modelo animal de Diabetes Mellitus tipo 2.

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dc.contributor.authorMega, C.
dc.contributor.authorVala, Helena
dc.contributor.authorTeixeira de Lemos, E.
dc.contributor.authorFernandes, R.
dc.contributor.authorGarrido, P.
dc.contributor.authorSereno, J.
dc.contributor.authorParada, B.
dc.contributor.authorMelo, F.
dc.contributor.authorTeixeira, F.
dc.contributor.authorReis, F.
dc.date.accessioned2013-01-07T19:40:01Z
dc.date.available2013-01-07T19:40:01Z
dc.date.issued2010
dc.description.abstractIntroduction: Type 2 diabetes mellitus 2 (T2DM) results, amongst others, from insulin resistance and reduction of ß-cell secretion, produced by functional changes and cellular mass reduction. Aim: Chronological morphologic characterization of the endocrine pancreas in an animal model of T2DM, the Zucker Diabetic Fatty (ZDF) rats to elucidate the underlying mechanisms of disease evolution. Material and methods: The pancreas of diabetic, obese rats (ZDF Gmi fa/fa) and their littermates (ZDF Gmi +/+) were compared at 8, 20, 26 weeks of age. After routine stain haematoxylin-eosin. Quantitative and semi-quantitative parameters were applied for evaluation of morphological changes in the islets of Langerhans. Quantitative parameters included counting islet numbers and islet size measurement. Semi-quantitive parameters consisted in the evaluation of islet conformation and perimeter regularity. The inflammatory lesions, vascular congestion and β-cell vacuolization were also classified in a scale from 0 to III. Results: Obese, diabetic ZDF (fa/fa) rats presented, with increasing age, a reduction in islet numbers, progressive deformation of insular architecture, fibrosis and vascular congestion, connected with inflammatory changes and joined by a decrease in insular cellular mass. Conclusion: The coincidence of the progressive histopathologic changes with the metabolic profile obtained in our previous studies shows a decrease in the capacity of the endocrine pancreas to compensate insulin resistance and ß-cell dysfunction. These two conjunct facts make the ZDF fa/fa, rat an appropriate model for a variety of T2DM studies.por
dc.identifier.citationMega C, Vala H, Teixeira de Lemos E, Fernandes R, Garrido P, Sereno J, Parada B, Melo F, Teixeira F, Reis F (2010). Utilização de parâmetros quantitativos e semi-quantitativos para avaliação dos Ilhéus de Langerhans num modelo animal de Diabetes Mellitus tipo 2. Revista Portuguesa de Ciências Veterinárias. Supl. 109(573-576): 82.por
dc.identifier.urihttp://hdl.handle.net/10400.19/1458
dc.language.isoporpor
dc.peerreviewedyespor
dc.subjectType 2 diabetes mellitus 2 (T2DM)por
dc.subjectmorphologic characterization of the endocrine pancreaspor
dc.subjectZucker Diabetic Fatty (ZDF) ratspor
dc.subjectanimal model of T2DMpor
dc.titleUtilização de parâmetros quantitativos e semi-quantitativos para avaliação dos Ilhéus de Langerhans num modelo animal de Diabetes Mellitus tipo 2.por
dc.typeconference object
dspace.entity.typePublication
oaire.citation.startPage82por
oaire.citation.titleRevista Portuguesa de Ciências Veterinárias.por
oaire.citation.volume109por
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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