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Rabbit thymic tissue – a potential marker for apoptosis techniques.

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dc.contributor.authorVala, Helena
dc.contributor.authorSantos, C.
dc.contributor.authorCruz, R.
dc.contributor.authorNóbrega, C.
dc.contributor.authorOrtiz, A. L.
dc.contributor.authorMesquita, JR
dc.contributor.authorEsteves, F.
dc.contributor.authorMega, A.
dc.date.accessioned2014-04-11T13:26:50Z
dc.date.available2014-04-11T13:26:50Z
dc.date.issued2013
dc.description.abstractBackground: Apoptosis detection methods have been proposed for diagnostic purposes, in particular for determining the biological characteristics of tumours and, indirectly, their prognosis. Implementation of a new technique requires the use of an effective positive control for correct assessment. Most specialty publications on the subject, do not indicate the positive controls used and do not disclose the obtained results therein. Objective: The aim of this work was to implement three different methods for the detection of apoptosis, using three canine tissue samples, consisting of breast cancer - carcinoma in situ, thymus and plasmacytoma; and a sample of rabbit tissues - thymus. Key words: Rabbit, thymus, immunohistochemistry, apoptosis, TUNEL, Bcl-2, Bax. Methods: Immunohistochemistry was performed, using pro- and antiapoptotic proteins, respectively, Bax and Bcl2 (1: 100; Santa Cruz, Biotechnology) and the TUNEL method (1: 2,5; In situ Cell Death Kit, Roche). Results Canine plasmacytoma revealed moderate positive reaction with all methods. Canine carcinoma in situ showed moderate positive reaction with pro-apoptotic antibodies and more intense with anti-apoptotic antibody. Dog’s thymus showed moderate positive reaction in cortical thymocytes with pro-apoptotic antibodies and weak in the medullary thymocytes. With the anti-apoptotic antibody, medullary thymocytes showed weak reaction and negativity in the cortex. Identical results were obtained in rabbit’s thymus, but with stronger intensity. Conclusions The rabbit thymus showed the most specific reaction of the tissues studied, by which the authors recommended it as a viable positive control for apoptosis studies.por
dc.description.sponsorshipFCT and CI&DETS (PEst-OE/CED/UI4016/2011)por
dc.identifier.citationVala H, Santos C, Cruz R, Nóbrega C, Ortiz AL, Mesquita JR, Esteves F, Mega A, (2013). Rabbit thymic tissue – a potential marker for apoptosis techniques. Revista Portuguesa de Ciências Veterinárias. 108 (585-586): 86-87.por
dc.identifier.urihttp://hdl.handle.net/10400.19/2072
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectRabbitpor
dc.subjectthymuspor
dc.subjectimmunohistochemistrypor
dc.subjectapoptosispor
dc.subjectTUNELpor
dc.subjectBcl-2por
dc.subjectBaxpor
dc.titleRabbit thymic tissue – a potential marker for apoptosis techniques.por
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboapor
oaire.citation.endPage87por
oaire.citation.startPage86por
oaire.citation.titleRevista Portuguesa de Ciências Veterinárias. 108 (585-586): 77-78.por
oaire.citation.volume108 (585-586)por
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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