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Advisor(s)
Abstract(s)
Objective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model.
Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium.
Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%).
Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans.
Description
Keywords
Bladder cancer Urinary bladder tumours N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)
Citation
Ana Isabel Faustino-Rocha, Carla Pinto, Helena Vala, Rita Ferreira, Paula Alexandra Oliveira (2014). Histological classification of urothelial lesions chemically-induced in the male rat model. Virchows Arch 465 (Supll I): S161 IF 2012 2.676