Repository logo
 
Publication

Histological classification of urothelial lesions chemically-induced in the male rat model

dc.contributor.authorFaustino-Rocha, Ana Isabel
dc.contributor.authorPinto, Carla
dc.contributor.authorVala, Helena
dc.contributor.authorFerreira, Rita
dc.contributor.authorOliveira, Paula Alexandra
dc.date.accessioned2015-03-23T11:22:30Z
dc.date.available2015-03-23T11:22:30Z
dc.date.issued2014
dc.description.abstractObjective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium. Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%). Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans.por
dc.description.sponsorshipFCT (PTDC/DES/114122/2009) and CI&DETS (PEst-OE/CED/UI4016)por
dc.identifier.citationAna Isabel Faustino-Rocha, Carla Pinto, Helena Vala, Rita Ferreira, Paula Alexandra Oliveira (2014). Histological classification of urothelial lesions chemically-induced in the male rat model. Virchows Arch 465 (Supll I): S161 IF 2012 2.676por
dc.identifier.urihttp://hdl.handle.net/10400.19/2730
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectBladder cancerpor
dc.subjectUrinary bladder tumourspor
dc.subjectN-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)por
dc.titleHistological classification of urothelial lesions chemically-induced in the male rat modelpor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.titleVirchows Archpor
oaire.citation.volume465 (Supll I)por
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
50 Urothelial lesions ESP 14.pdf
Size:
1.69 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Item-specific license agreed upon to submission
Description: