Publication
Histological classification of urothelial lesions chemically-induced in the male rat model
| dc.contributor.author | Faustino-Rocha, Ana Isabel | |
| dc.contributor.author | Pinto, Carla | |
| dc.contributor.author | Vala, Helena | |
| dc.contributor.author | Ferreira, Rita | |
| dc.contributor.author | Oliveira, Paula Alexandra | |
| dc.date.accessioned | 2015-03-23T11:22:30Z | |
| dc.date.available | 2015-03-23T11:22:30Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Objective: Bladder cancer is one of the most frequent of the urinary tract. Urinary bladder tumours induced by the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) are similar to those observed in humans. This work aimed to classify, by histology, the urinary bladder tumours BBN-induced in a rat model. Methods: Procedures were made in accordance with the Directive 2010/63/EU. Eleven Wistar male rats with five weeks of age were used. BBN was administered in drinking water at a concentration of 0.05% water during the first 20 weeks of the protocol. Thirty-five weeks after the start of the protocol, animals were humanely sacrificed by the overdose of pentobarbital sodium. Results: No animals died during the protocol. In the histological analysis of the urinary bladders were identified the following lesions: simple hyperplasia (81.8%), nodular hyperplasia (54.5%), papillary hyperplasia (14%), papilloma (45.4%), papillary neoplasm of low malignant potential (36.4%), low-grade papillary carcinoma (90.9%), high-grade papillary carcinoma (72.7%), invasive carcinoma (14%) and epidermoid metaplasia with keratinization (45.4%). Conclusion: Our team has a vast experience on the bladder cancer animal models; however this is the first protocol in male rats. We concluded that the lesions identified are similar to those observed in Humans. | por |
| dc.description.sponsorship | FCT (PTDC/DES/114122/2009) and CI&DETS (PEst-OE/CED/UI4016) | por |
| dc.identifier.citation | Ana Isabel Faustino-Rocha, Carla Pinto, Helena Vala, Rita Ferreira, Paula Alexandra Oliveira (2014). Histological classification of urothelial lesions chemically-induced in the male rat model. Virchows Arch 465 (Supll I): S161 IF 2012 2.676 | por |
| dc.identifier.uri | http://hdl.handle.net/10400.19/2730 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.subject | Bladder cancer | por |
| dc.subject | Urinary bladder tumours | por |
| dc.subject | N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) | por |
| dc.title | Histological classification of urothelial lesions chemically-induced in the male rat model | por |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Virchows Arch | por |
| oaire.citation.volume | 465 (Supll I) | por |
| person.familyName | Vala Correia | |
| person.givenName | Helena Maria | |
| person.identifier.ciencia-id | 7A1E-E85E-FFA4 | |
| person.identifier.orcid | 0000-0001-6829-4867 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | conferenceObject | por |
| relation.isAuthorOfPublication | cdc3d2e2-df06-40ed-8900-1ecbc8a06c8a | |
| relation.isAuthorOfPublication.latestForDiscovery | cdc3d2e2-df06-40ed-8900-1ecbc8a06c8a |