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Orientador(es)
Resumo(s)
The authors proposed a development of a semiquantitative morphological assessment to intestinal mucosa edema, congestion, hyperemia, hemorrhage, inflammatory infiltration, cellular degeneration, necrosis, epithelial detachment and also a quantitative morphological assessment to evaluate mucosal loss (ML) percentage and mucosal crypt:interstitium ratio (C:I) in pigs submitted to severe hypotension.
The semiquantitative parameters edema, congestion, hyperemia, hemorrhage, inflammatory infiltration, cellular degeneration, necrosis were evaluated by two independent examiners and classified in a specific scale from 0 to 3, according to Çetin et al., (1995). The epithelial detachment was evaluated by two independent examiners and registered in a specific scale from 0 to 5, according to Chiu et al., (1970) and Kaplan et al., (2007).
The quantitative morphological assessment to ML percentage and mucosal C:I was achieved using an ocular 1000 micrometric reticule divisions across 10 randomly selected microscopic fields in each section, using a10x objective, adapted from Faleiros et al., (2001).
Descrição
Palavras-chave
semiquantitative morphological assessment intestinal lesions percentage and mucosal crypt:interstitium ratio (C:I) mucosal loss (ML) pig
Contexto Educativo
Citação
Oliveira AL, Ferreira DA, Venâncio C, Silva A, Sousa A, Amorim P, Antunes LM, Vala H (2010). Morfometria Intestinal em Suínos. Revista Portuguesa de Ciências Veterinárias. Supl. 109(573-576): 80.