| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 61.41 KB | Adobe PDF |
Advisor(s)
Abstract(s)
The authors proposed a development of a semiquantitative morphological assessment to intestinal mucosa edema, congestion, hyperemia, hemorrhage, inflammatory infiltration, cellular degeneration, necrosis, epithelial detachment and also a quantitative morphological assessment to evaluate mucosal loss (ML) percentage and mucosal crypt:interstitium ratio (C:I) in pigs submitted to severe hypotension.
The semiquantitative parameters edema, congestion, hyperemia, hemorrhage, inflammatory infiltration, cellular degeneration, necrosis were evaluated by two independent examiners and classified in a specific scale from 0 to 3, according to Çetin et al., (1995). The epithelial detachment was evaluated by two independent examiners and registered in a specific scale from 0 to 5, according to Chiu et al., (1970) and Kaplan et al., (2007).
The quantitative morphological assessment to ML percentage and mucosal C:I was achieved using an ocular 1000 micrometric reticule divisions across 10 randomly selected microscopic fields in each section, using a10x objective, adapted from Faleiros et al., (2001).
Description
Keywords
semiquantitative morphological assessment intestinal lesions percentage and mucosal crypt:interstitium ratio (C:I) mucosal loss (ML) pig
Citation
Oliveira AL, Ferreira DA, Venâncio C, Silva A, Sousa A, Amorim P, Antunes LM, Vala H (2010). Morfometria Intestinal em Suínos. Revista Portuguesa de Ciências Veterinárias. Supl. 109(573-576): 80.