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Advisor(s)
Abstract(s)
Background: Human noroviruses are the major cause of nonbacterial epidemic gastroenteritis worldwide. Although noroviruses have been absent from the list of gastrointestinal infectious agents in dogs, recently several studies emphasized their role in the aetiology of gastroenteritis, suggesting the possibility of a novel model for human infection.
The aim of this study was to evaluate the histopathological lesions in gastroenteritis, associated to canine norovirus, in order to contribute to the knowledge of its pathogenic features and the role of apoptotic events in the noroviruses pathogenesis.
Material and methods: Samples of small intestine were collected from 2 adult dogs (2-year-old cross-bred and a 6 year-old cocker spaniel) housed in a kennel where CaNoV had been diagnosed.
Samples of small intestine were collected and fixed (10% neutral buffered formalin) for a maximum of 48 hours, dehydrated (graded ethanol series) and embedded in paraffin wax.
Apoptosis studies combined the immunohistochemical detection of cytochrome c (anti-cytochrome C sc-8385 antibody, Santa Cruz Biotechnology) and the DNA fragmentation by TUNEL assay (In Situ Cell Death Detection Kit, Roche, Germany).
Samples were examined by light microscopy (Microscope Zeiss Mod. Axioplan 2), by two independent observers.
All procedures were performed in accordance with the European Communities Council Directive 86/609/ECC.
Results: The microscopic findings revealed severe epithelial detachment, mucosal loss and hypertrophy of all small intestinal layers. Also, an intense mononuclear inflammatory infiltrate within lamina propria of small intestine proximal segments were seen, very similar to IBD lesional pattern.
Discussion and Conclusions: Canine inflammatory bowel disease (IBD) is a heterogeneous group of chronic gastrointestinal disorders with unknown aetiology, immunologically-mediated, similar to human IBD. With this study it is tempting to hypothesize that canine norovirus could be significantly evolved in the complex aetiopathogenesis of IBD, identically in human Crohn disease - an example of human IBD, which could highlight the relevance of norovirus, in its role of in the aetiopathogenesis of this chronic condition.
Description
Keywords
histopathologic lesions gastroenteritis canine norovirus
Pedagogical Context
Citation
Vala H, Mesquita JR, Nascimento MSJ (2015). Characterization of histopathologic lesions in gastroenteritis associated to norovirus in a model of study. Virchows Arch 467 (Supll I): S120