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Advisor(s)
Abstract(s)
BACKGROUND:
E-cadherin and β-catenin are adhesion molecules that promote integrity and stability of the urothelium. A decrease in theirexpression is associated with more aggressive tumour phenotypes with the ability to invade and metastasize.
MATERIAL AND METHODS:
45 ICR male mice were used, of which 25 received N-butyl-N-(4-hydroxybutyl)nitrosamine (0.05%) in drinking water for a period of 12 weeks. Immunohistochemical expression was evaluated in all urinary bladder preparations for E-cadherin and for β-catenin.
RESULTS:
Preneoplastic lesions showed staining patterns similar to normal urothelium. In simple and nodular hyperplasia, membrane staining was dominant (66.7-78.6 and 50-100%, respectively). In dysplasia a cytoplasmic pattern was prevalent (86.7-100%). Neoplastic lesions exhibit an abnormal staining pattern (100%) with heterogeneous staining (cytoplasmic, nuclear and membrane staining). A strong correlation was observed between both adhesion molecule staining patterns (r = 0.83; p = 0.039).
CONCLUSIONS:
In mice, as in humans, E-cadherin and β-catenin are valuable tools to investigate cellular adhesion status of urothelium and can be considered as indicators of tumour aggressiveness and evolution.
Description
Keywords
E-cadherin β-catenin urothelium mice N-butyl-N-(4-hydroxybutyl)nitrosamine
Citation
Vasconcelos-Nóbrega, C., Costa, C., Vala, H., Colaço, A., Santos, L., Lopes, C., & Oliveira, P. A. (2013). E-cadherin and β-catenin expression during urothelial carcinogenesis induced by N-butyl-N-(4-hydroxybutyl) nitrosamine in mice. Urologia Internationalis, 91(4), 462–466. http://doi.org/10.1159/000348329