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Advisor(s)
Abstract(s)
Introduction: Apoptosis plays an important role in cellular damage caused by acute renal hypoperfusion. The aim of this study was to detect apoptotic events in an animal model of acute haemorrhage, followed by volume replacement with different intravenous solutions.
Materials and Methods: Renal samples were collected from animals submitted to passive arterial blood bleeding and reperfusion with a crystalloid (RL) (G1) and with a synthetic colloid (HES 130/0.4) (G2). All procedures were carried out under personal and project licenses approved by the Ethical Committee of the national regulatory office. Immunohistochemistry was performed, using cytochrome c antibody to detect mitochondrial activity and in situ TUNEL method to evaluate endonucleosomic cleavage of DNA by TdT. An immunofluorescence method, the M30 Cytodeath, was also used to detect early apoptotic events.
Results: In all groups, apoptosis was detected in the epithelial tubular cells of the proximal and distal convoluted tubules, in the loop of Henle and in the collecting tubules. However, the percentage of apoptotic cells and the intensity of reaction was significantly higher in G2.
Conclusions: Apoptosis plays an important role in the cellular damage in renal tubules following acute haemorrhage and volume replacement. The type of solution used for volume replacement may influence the extent of renal damage resulting from this clinical situation. In the present study, the use of RL was related with decreased cytosolic cytochrome c and a lower apoptotic index in the tubular renal cells.
Description
Keywords
APOPTOTIC EVENTS ACUTE HAEMORRHAGE
Citation
H Vala, R Cruz, C Venâncio, C Garcia, A Silva, J Mesquita, AL Ortiz, D Ferreira (2016). Detection of apoptotic events, using different methods, in renal tissues after acute haemorrhage. Journal of Comparative Pathology. Volume 154: 79