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Detection of apoptotic events, using different methods, in renal tissues after acute haemorrhage

dc.contributor.authorVala, Helena
dc.contributor.authorCruz, R.
dc.contributor.authorVenâncio, C.
dc.contributor.authorGarcia, C.
dc.contributor.authorSilva, A.
dc.contributor.authorMesquita, J.
dc.contributor.authorOrtiz, A. L.
dc.contributor.authorFerreira, D.
dc.date.accessioned2016-02-17T15:32:11Z
dc.date.available2016-02-17T15:32:11Z
dc.date.issued2015
dc.description.abstractIntroduction: Apoptosis plays an important role in cellular damage caused by acute renal hypoperfusion. The aim of this study was to detect apoptotic events in an animal model of acute haemorrhage, followed by volume replacement with different intravenous solutions. Materials and Methods: Renal samples were collected from animals submitted to passive arterial blood bleeding and reperfusion with a crystalloid (RL) (G1) and with a synthetic colloid (HES 130/0.4) (G2). All procedures were carried out under personal and project licenses approved by the Ethical Committee of the national regulatory office. Immunohistochemistry was performed, using cytochrome c antibody to detect mitochondrial activity and in situ TUNEL method to evaluate endonucleosomic cleavage of DNA by TdT. An immunofluorescence method, the M30 Cytodeath, was also used to detect early apoptotic events. Results: In all groups, apoptosis was detected in the epithelial tubular cells of the proximal and distal convoluted tubules, in the loop of Henle and in the collecting tubules. However, the percentage of apoptotic cells and the intensity of reaction was significantly higher in G2. Conclusions: Apoptosis plays an important role in the cellular damage in renal tubules following acute haemorrhage and volume replacement. The type of solution used for volume replacement may influence the extent of renal damage resulting from this clinical situation. In the present study, the use of RL was related with decreased cytosolic cytochrome c and a lower apoptotic index in the tubular renal cells.pt_PT
dc.identifier.citationH Vala, R Cruz, C Venâncio, C Garcia, A Silva, J Mesquita, AL Ortiz, D Ferreira (2016). Detection of apoptotic events, using different methods, in renal tissues after acute haemorrhage. Journal of Comparative Pathology. Volume 154: 79pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.19/3062
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationFCT, FCOMP-01-0124-FEDER-009525; IPV, CI&DETS, FCT and QREN/FEDER (Ovislab ICT-2013-05-004-5314 ID-64757)pt_PT
dc.subjectAPOPTOTIC EVENTSpt_PT
dc.subjectACUTE HAEMORRHAGEpt_PT
dc.titleDetection of apoptotic events, using different methods, in renal tissues after acute haemorrhagept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.titleJournal of Comparative Pathologypt_PT
oaire.citation.volume154pt_PT
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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