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Advisor(s)
Abstract(s)
The aim of this study was to evaluate the possibility of using some antibodies as diagnostic instruments, in Bovine Ocular Squamous Cell Carcinoma (BOSCC) and establishing the relationship between immunohistochemical results and the degree of differentiation.
Thirty two samples from nineteen bovine ocular tumours were collected at the Matadouro Frigorífico e Industrial of Ponta Delgada, S. Miguel Island, Azores. Material was fixed in 10% formalin, embedded in paraffin and stained for routine histopathological diagnosis for tumour classification.
Diagnosis of BOSCC was confirmed in all samples. Nine carcinomas were well differentiated, five moderately differentiated and five non-differentiated.
Immunohistochemical indirect technique Avidin-Biotin-Peroxidase was applied. Two antibodies against distinct keratins were used: a monoclonal antibody MNF116, anti-cytokeratins 5, 6, 8, 17 and 19 (DAKO), characteristic of non differentiated keratinocytes and simple epithelia, and a monoclonal antibody LP34, anti-cytokeratins 5, 6 and 18 (DAKO), characteristic of well-differentiated keratinocytes.
Epidermal differentiation markers were also used: a monoclonal antibody anti-human involucrin, for keratinocytes of the stratum spinosum and stratum granulosum and a polyclonal antibody anti-human profilaggrin (ZYMED), for keratinocytes of the stratum granulosum.
Most antibodies, without exception, reacted with the various types of BOSCC;
The antibody MNF 116 was positive in all carcinomas, independently of their degree of differentiation. The antibody LP34 was positive in all carcinomas, except for one in each differentiation grade group.
The anti involucrin antibody was positive in all but one non-differentiated carcinomas. The anti profilaggrin antibody was positive in only two moderately differentiated carcinomas, in all but one well-differentiated carcinomas, and in all but one non-differentiated carcinomas.
Description
Keywords
Bovine Ocular Squamous Cell Carcinoma BOSCC immunohistochemistry histopathological diagnosis MNF116, anti-cytokeratins 5, 6, 8, 17 and 19 LP34, anti-cytokeratins 5, 6 and 18 involucrin profilaggrin
Pedagogical Context
Citation
Vala H, Fondevila D, Carvalho T, Pinto C, Peleteiro C, Pinho M, Ferrer L (2002). Immunohistochemistry of epidermal differentiation markers in bovine ocular squamous cell carcinoma and distribution of cytokeratins. Revista Portuguesa de Ciências Veterinárias. Supl. 118: 34-35