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Immunohistochemistry of epidermal differentiation markers in bovine ocular squamous cell carcinoma and distribution of cytokeratins

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dc.contributor.authorVala, Helena
dc.contributor.authorFondevila, D.
dc.contributor.authorCarvalho, T.
dc.contributor.authorPinto, C.
dc.contributor.authorPeleteiro, C.
dc.contributor.authorPinho, M.
dc.contributor.authorFerrer, L.
dc.date.accessioned2013-01-07T18:28:03Z
dc.date.available2013-01-07T18:28:03Z
dc.date.issued2002
dc.description.abstractThe aim of this study was to evaluate the possibility of using some antibodies as diagnostic instruments, in Bovine Ocular Squamous Cell Carcinoma (BOSCC) and establishing the relationship between immunohistochemical results and the degree of differentiation. Thirty two samples from nineteen bovine ocular tumours were collected at the Matadouro Frigorífico e Industrial of Ponta Delgada, S. Miguel Island, Azores. Material was fixed in 10% formalin, embedded in paraffin and stained for routine histopathological diagnosis for tumour classification. Diagnosis of BOSCC was confirmed in all samples. Nine carcinomas were well differentiated, five moderately differentiated and five non-differentiated. Immunohistochemical indirect technique Avidin-Biotin-Peroxidase was applied. Two antibodies against distinct keratins were used: a monoclonal antibody MNF116, anti-cytokeratins 5, 6, 8, 17 and 19 (DAKO), characteristic of non differentiated keratinocytes and simple epithelia, and a monoclonal antibody LP34, anti-cytokeratins 5, 6 and 18 (DAKO), characteristic of well-differentiated keratinocytes. Epidermal differentiation markers were also used: a monoclonal antibody anti-human involucrin, for keratinocytes of the stratum spinosum and stratum granulosum and a polyclonal antibody anti-human profilaggrin (ZYMED), for keratinocytes of the stratum granulosum. Most antibodies, without exception, reacted with the various types of BOSCC; The antibody MNF 116 was positive in all carcinomas, independently of their degree of differentiation. The antibody LP34 was positive in all carcinomas, except for one in each differentiation grade group. The anti involucrin antibody was positive in all but one non-differentiated carcinomas. The anti profilaggrin antibody was positive in only two moderately differentiated carcinomas, in all but one well-differentiated carcinomas, and in all but one non-differentiated carcinomas.por
dc.identifier.citationVala H, Fondevila D, Carvalho T, Pinto C, Peleteiro C, Pinho M, Ferrer L (2002). Immunohistochemistry of epidermal differentiation markers in bovine ocular squamous cell carcinoma and distribution of cytokeratins. Revista Portuguesa de Ciências Veterinárias. Supl. 118: 34-35por
dc.identifier.urihttp://hdl.handle.net/10400.19/1431
dc.language.isoporpor
dc.peerreviewedyespor
dc.subjectBovine Ocular Squamous Cell Carcinomapor
dc.subjectBOSCCpor
dc.subjectimmunohistochemistrypor
dc.subjecthistopathological diagnosispor
dc.subjectMNF116, anti-cytokeratins 5, 6, 8, 17 and 19por
dc.subjectLP34, anti-cytokeratins 5, 6 and 18por
dc.subjectinvolucrinpor
dc.subjectprofilaggrinpor
dc.titleImmunohistochemistry of epidermal differentiation markers in bovine ocular squamous cell carcinoma and distribution of cytokeratinspor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.endPage35por
oaire.citation.startPage34por
oaire.citation.titleRevista Portuguesa de Ciências Veterinárias.por
oaire.citation.volume118por
person.familyNameVala Correia
person.givenNameHelena Maria
person.identifier.ciencia-id7A1E-E85E-FFA4
person.identifier.orcid0000-0001-6829-4867
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublicationcdc3d2e2-df06-40ed-8900-1ecbc8a06c8a
relation.isAuthorOfPublication.latestForDiscoverycdc3d2e2-df06-40ed-8900-1ecbc8a06c8a

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