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Advisor(s)
Abstract(s)
The purpose of the present study was to immunocharacterize Bovine Ocular Squamous Cell Carcinoma neoplastic cells, in terms of proliferation index and DNA damage, using two human primary antibodies anti- Ki67 antigen of the cell cycle and anti- p53.
Ki67 is a nuclear antigen expressed in dividing cells during late G1, G2, S-phase and M-phase that disapears rapidly after mitosis. p53 is a tumor supressor protein that plays a key role in the control of the DNA damage acting as a supressor of tumour cell proliferation, enhancing genetic stability. Mutation of the gene that codes for p53 provides an extended intracellular half-life of the protein and greater stability, therefore increasing its expression.
Tumours for this study were obtained at the slautherhouse, in Ponta Delgada, Azores. A total of thirty samples were colected from eighteen bovine. Immunohistochemical analysis was performed in parafin embeded sections of formalin fixed material, using the Avidin-Biotin-Peroxidase method (ABC). Primary antibodies used were anti-human p53 (NCL – p53 CM-1, NOVOCASTRA, UK) and anti-human Ki67 (NCL – Ki67p, NOVOCASTRA, UK), both policlonal.
Positivity for both Ki67 and p53 was evaluated quantitatively and semi-quantitatively, respectively
Description
Keywords
Bovine Ocular Squamous Cell Carcinoma BOSCC immunohistochemistry histopathological diagnosis Ki67 p53
Citation
Carvalho T, Vala H, Fondevila D, Pinto C, Peleteiro C, Pinho M, Ferrer L (2002). Immunohistochemical studies of proliferation cell markers and DNA mutation in bovine ocular squamous cell carcinoma. Revista Portuguesa de Ciências Veterinárias. Supl. 118: 35