Publication
Immunohistochemical studies of proliferation cell markers and DNA mutation in bovine ocular squamous cell carcinoma
dc.contributor.author | Carvalho, T. | |
dc.contributor.author | Vala, Helena | |
dc.contributor.author | Fondevila, D. | |
dc.contributor.author | Pinto, C. | |
dc.contributor.author | Peleteiro, C. | |
dc.contributor.author | Pinho, M. | |
dc.contributor.author | Ferrer, L. | |
dc.date.accessioned | 2013-01-07T18:32:44Z | |
dc.date.available | 2013-01-07T18:32:44Z | |
dc.date.issued | 2002 | |
dc.description.abstract | The purpose of the present study was to immunocharacterize Bovine Ocular Squamous Cell Carcinoma neoplastic cells, in terms of proliferation index and DNA damage, using two human primary antibodies anti- Ki67 antigen of the cell cycle and anti- p53. Ki67 is a nuclear antigen expressed in dividing cells during late G1, G2, S-phase and M-phase that disapears rapidly after mitosis. p53 is a tumor supressor protein that plays a key role in the control of the DNA damage acting as a supressor of tumour cell proliferation, enhancing genetic stability. Mutation of the gene that codes for p53 provides an extended intracellular half-life of the protein and greater stability, therefore increasing its expression. Tumours for this study were obtained at the slautherhouse, in Ponta Delgada, Azores. A total of thirty samples were colected from eighteen bovine. Immunohistochemical analysis was performed in parafin embeded sections of formalin fixed material, using the Avidin-Biotin-Peroxidase method (ABC). Primary antibodies used were anti-human p53 (NCL – p53 CM-1, NOVOCASTRA, UK) and anti-human Ki67 (NCL – Ki67p, NOVOCASTRA, UK), both policlonal. Positivity for both Ki67 and p53 was evaluated quantitatively and semi-quantitatively, respectively | por |
dc.description.sponsorship | The present work was financed by PROGRAMA PRAXIS XXI, Fundação para a Ciência e Tecnologia, Ministério de Ciência e Tecnologia, Portugal and Centro Interdisciplinar de Investigação em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa | por |
dc.identifier.citation | Carvalho T, Vala H, Fondevila D, Pinto C, Peleteiro C, Pinho M, Ferrer L (2002). Immunohistochemical studies of proliferation cell markers and DNA mutation in bovine ocular squamous cell carcinoma. Revista Portuguesa de Ciências Veterinárias. Supl. 118: 35 | por |
dc.identifier.uri | http://hdl.handle.net/10400.19/1434 | |
dc.language.iso | por | por |
dc.peerreviewed | yes | por |
dc.subject | Bovine Ocular Squamous Cell Carcinoma | por |
dc.subject | BOSCC | por |
dc.subject | immunohistochemistry | por |
dc.subject | histopathological diagnosis | por |
dc.subject | Ki67 | por |
dc.subject | p53 | por |
dc.title | Immunohistochemical studies of proliferation cell markers and DNA mutation in bovine ocular squamous cell carcinoma | por |
dc.type | conference object | |
dspace.entity.type | Publication | |
oaire.citation.endPage | 35 | por |
oaire.citation.title | Revista Portuguesa de Ciências Veterinárias | por |
oaire.citation.volume | 118 | por |
person.familyName | Vala Correia | |
person.givenName | Helena Maria | |
person.identifier.ciencia-id | 7A1E-E85E-FFA4 | |
person.identifier.orcid | 0000-0001-6829-4867 | |
rcaap.rights | restrictedAccess | por |
rcaap.type | conferenceObject | por |
relation.isAuthorOfPublication | cdc3d2e2-df06-40ed-8900-1ecbc8a06c8a | |
relation.isAuthorOfPublication.latestForDiscovery | cdc3d2e2-df06-40ed-8900-1ecbc8a06c8a |
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