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- Investigação e formação em questões de género com criançasPublication . Almeida, Ana Margarida; Cardoso, Ana Paula; Lacerda José, Carla Sofia Pereira; Ferreira, Manuela; Margarida Campos, Sofia; de Pinho Ferreira Guiné, RaquelIntrodução: As questões de género são um tema fundamental na atualidade, que importa debater desde cedo, a fim de contribuir para o desenvolvimento de uma cidadania plena. Objetivo: Compreender as perspetivas das crianças sobre a igualdade de género, em geral e a nível profissional e salarial, antes e depois de uma intervenção pedagógica interdisciplinar no 1.º Ciclo do Ensino Básico (CEB). Métodos: Estudo qualitativo que consistiu numa investigação sobre as práticas, utilizando diferentes técnicas de recolha de dados. Participaram 24 crianças, do 4.º ano, com idades compreendidas entre os 9 e 11 anos, de uma escola pública de um agrupamento de escolas do concelho de Viseu (Portugal). Resultados: Os dados revelaram que as crianças tinham já conhecimentos prévios sobre o assunto na linha do que a sociedade veicula. Todavia, a intervenção realizada levou a que muitas delas modificassem as suas perspetivas e se tornassem mais conscientes da influência de alguns estereótipos de género. As crianças manifestaram espanto pela diferença salarial entre homens e mulheres, sobre a qual não tinham ainda refletido e, em geral, demonstraram concordância em haver direitos iguais para todos no meio social e profissional. Conclusão: A educação para a igualdade de género é essencial em todo o percurso educativo. Com ela, as crianças têm a possibilidade de desconstruir preconceitos enraizados na sociedade, de desenvolver a sua personalidade de forma integral e de exercer os seus direitos cívicos com assertividade no futuro.
- Nutritional Approaches in Neurodegenerative Disorders: A Mini Scoping Review with Emphasis on SPG11-Related ConditionsPublication . Ribeiro, Paulo Renato; Ferreira, Carmen; Antunes, Carlos; Dias, Gonçalo; Cunha Silva Reis Lima, Maria Joao; de Pinho Ferreira Guiné, Raquel; Teixeira-Lemos, EditeBackground: Neurodegenerative diseases, including spastic paraplegia type 11 (SPG11), are complex disorders characterized by progressive neurological decline and significant metabolic disturbances. Spatacsin, the protein encoded by the SPG11 gene, plays a critical role in autophagy and lysosomal homeostasis, which are essential for neuronal health. Its impairment leads to defective cellular clearance and neurodegeneration. Recently, personalized and precision nutrition have emerged as promising approaches to enhance clinical outcomes by tailoring dietary interventions to individual genetic, metabolic, and phenotypic profiles. Objectives: This mini scoping review aimed to synthesize current evidence on the application of personalized and precision nutrition in SPG11 and to explore how insights from related neurodegenerative diseases could inform the development of future dietary and metabolic interventions for this rare disorder. Methods: Following PRISMA-ScR guidelines, a scoping review was conducted using PubMed, Scopus, and Web of Science databases (2020–2024). Eligible studies included investigations addressing nutritional, genomic, or metabolic interventions in neurodegenerative diseases. Of 30 screened papers, nine met the inclusion criteria, primarily focusing on nutritional and metabolic interventions related to neurodegenerative and neuromuscular conditions. Results: To date, no dietary intervention trials have been conducted specifically for SPG11. However, evidence from studies on related neurodegenerative diseases suggests that antioxidant, mitochondrial-supportive, and microbiota-targeted dietary approaches may beneficially influence key pathological processes such as oxidative stress, lipid dysregulation, and autophagy—core mechanisms that are also central to SPG11 pathophysiology. Conclusions: Although current evidence remains preliminary, personalized nutrition is a promising supplementary strategy for managing neurodegenerative diseases, including SPG11. Future research should incorporate systems-based approaches that combine dietary, metabolic, and neuroimaging assessments, with sex and comorbidity-stratified analyses, multi-omics profiling, and predictive modeling. These frameworks could help design safe, effective, and personalized nutritional interventions aimed at enhancing metabolic resilience and slowing disease progression in SPG11.
- PEF Priming of Seeds for Microgreen Production: A Comparative StudyPublication . Aguiar-Macedo, Mafalda; Dulyanska, Yuliya; Guiné, Raquel; Costa, Daniela V. T. A.; Redondod, Luis M; de Pinho Ferreira Guiné, RaquelSeed priming with Pulsed Electric Fields (PEF) is a promising strategy to enhance early plant development and crop quality. This study evaluated PEF priming effects on Beetroot (Beta vulgaris L.), Arugula (Eruca vesicaria), and Basil (Ocimum basilicum L.) microgreens by assessing the effects of three distinct monopolar PEF protocols (PEFA: 2 kV/cm; PEFB: 3 kV/cm; PEFC: 4 kV/cm). PEFB and PEFC treatments significantly influenced imbibition. Germination Indexes (DGI, CGI, and SVI) were positively and significantly affected, with radicle length increasing up to 33% and DGI improving from 40 to 66 on the 1st day (Beetroot, PEFC). Chlorophylls and the Total Carotenoid concentration increased in Basil but decreased in Beetroot and Arugula. Fat and Protein increased in Beetroot (Fat: +41%; Protein: +34%) and Arugula (Fat: +91%; Protein: +11%) treated with PEFC. PEFB led to an increase in Starch in all species. Crude Fibre and Neutral Detergent Fibre decreased amongst all species. Methionine rose by 100% in Beetroot treated with PEFC. Sensory analysis showed slight increases in Sweet (Beetroot) and Aroma Intensity (Basil and Arugula), although these changes were not statistically significant. Species-specific responses to different PEF protocols were observed: optimal protocols seem to be PEFC for Beetroot, PEFB for Arugula, and PEFA/B for Basil.
- Physicochemical and Microbiological Properties of Hazelnuts from Three Varieties Cultivated in PortugalPublication . Ferrão, Ana Cristina; Guiné, Raquel; Silva, Marco; Lopes, Arminda; Correia, PaulaHazelnut is an important crop worldwide, and the characteristics of the fruits are quite variable according to a number of factors, including variety and cultivation conditions, which in turn can vary according to harvest year. This study aimed to investigate some physical and chemical characteristics of three hazelnut varieties grown in Portugal (Grada de Viseu, Tonda di Giffoni and Butler) along two different harvesting years (2021 and 2022). Also, the microbial quality was investigated for its relevance to the conservation of the fruits. The physical properties evaluated were biometric characteristics and colour, the chemical components analysed were moisture, lipids, protein, ash and fibre, and the microbial properties investigated were the microorganisms, moulds and yeasts. The results showed that, generically, statistically significant differences were found between the three varieties under study on several properties investigated. The kernel was confirmed as the lighter part of all hazelnuts, and when comparing between varieties, Tonda di Giffoni presented the lighter fruits in both harvesting years. With respect to weight, the Tonda di Giffoni variety was the lightest in both harvest years. Moisture content was observed to be higher than the recommended limits for two of the samples (Grada de Viseu in 2021: 6.01 ± 0.26 g/100 g and Butler in 2022: 6.02 ± 0.37 g/100 g), although the difference was marginal given that the recommended limit is 6%. Not surprisingly, lipids were the major chemical component, ranging from 66.46 ± 1.67 to 70.14 ± 1.75 g/100 g in 2021 and from 64.38 ± 1.67 to 77.77 g/100 g in 2022. It was further observed that the three varieties presented a satisfactory microbiological quality. Finally, applying factor analysis with principal components and Varimax rotation, a solution that explains 92.8% of the variance was obtained. This study provided information that is relevant for the characterisation and evaluation of variability according to the year of hazelnuts of three varieties cultivated in Portugal.
- Television cooking shows and culinary programmes – factor analysis and cluster segmentationPublication . Guiné, Raquel; Florença, Sofia; Teixeira-Lemos, Edite; Lima, Maria JoãoThis study investigates the viewing habits regarding culinary television content, such as competition shows and recipe programmes, and their impact on eating out and cooking behaviours. Data were collected through a questionnaire survey in Portugal, on a sample of 250 adult citizens. Data analysis included basic statistics, factor and cluster analysis, and characterisation of the obtained clusters. Results showed that when the participants eat out, they usually do it in restaurants that serve national dishes or in fast food service providers. About two-thirds of the participants watch culinary programmes on TV, although with a low frequency, typically less than once a week, and especially at the end of the day. They believe these programmes can influence the diet because they constitute an opportunity to learn new recipes and new ways to prepare food, including reusing food leftovers to prepare innovative and creative meals. The study reveals that culinary programmes encourage viewers to cook more at home, prepare healthier meals, and reduce food waste. Factor analysis allowed the extraction of two factors, one linked to positive perceptions and the other to negative perceptions. Cluster analysis showed three groups of individuals: those who believe the culinary programmes and shows are not useful, those who find them educational and those who find them essentially entertaining. Finally, significant differences were observed between the clusters for variables such as self-evaluation of the health status, frequency of eating out, and fre- quency of cooking meals. In summary, this work allowed the identification of three differentiated patterns of individuals in relation to the visualisation of TV cooking shows or culinary programmes.